Quantification of tumour‐induced angiogenesis by image analysis
Quantification of tumour‐induced angiogenesis by image analysis
复制标题
通过图像分析量化肿瘤诱导的血管生成
DOI：
10.1046/j.1365-2613.1996.00970.x
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发表时间：
1996
影响因子：
3
通讯作者：
K. Okada
中科院分区：
文献类型：
--
作者：
M. Iwahana;Y. Nakayama;Noriko Tanaka;M. Goryo;K. Okada
使用图像分析仪开发了用于体内和体外血管生成的定量技术。在体内研究中，将填充有小鼠肉瘤180（S180）细胞的Millipore室皮下移植到小鼠的背侧，并通过图像分析定量肿瘤细胞诱导的新血管形成的面积。对比度差的血管网图像通过拉普拉斯变换得到对比度改善。无肿瘤细胞的对照组血管网面积为16.9 mm2，有肿瘤细胞的对照组血管网面积为44.2 mm2，表明肿瘤细胞的新生血管面积显著增加。 在体外研究中，用S180细胞的条件培养基诱导血管内皮细胞迁移。采用图像分析技术对迁移的内皮细胞进行细胞核自动计数，细胞核用姬姆萨氏液染成紫色。这种通过图像分析仪进行的自动测量预计将节省劳动力和时间。生物标记板分析显示，S180条件培养基诱导的内皮细胞迁移是由于趋化性。使用自动图像分析仪的定量方法在评价肿瘤诱导新生血管形成以及体内和体外药物对肿瘤血管生成的影响方面具有价值
Quantitative techniques for in vivo and in vitro angiogenesis were developed using an image analyser. In the in vivo study, a Millipore chamber filled with mouse sarcoma 180 (S180) cells was transplanted subcutaneously to the dorsal side of a mouse, and the area of neovascularization induced by the tumour cells was quantified by image analysis. Images of vascular networks with poor contrast had their contrast improved by Laplacean transformation. The area of vascular network was 16.9 mm2 in the control group without tumour cells and 44.2 mm2 in the group with tumour cells, demonstrating a significant increase in neovascularized area by tumour cells. In the in vitro study, migration of vascular endothelial cells was induced with conditioned media of S180 cells. Image analysis was used to count automatically the nuclei of migrated endothelial cells, which were stained violet with Giemsa's solution. This automated measurement by image analyser is expected to save labour and time. Checkerboard analysis revealed that the endothelial cell migration induced by S180‐conditioned medium was due to chemotaxis. The quantitation method using an automated image analyser is valuable in evaluating the induction of neovascularization by tumours and the effect of pharmacological agents on tumour angiogenesis in vivo and in vitro