Two-step protocol for isolation and culture of cardiospheres.
Two-step protocol for isolation and culture of cardiospheres.
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DOI：
10.1007/978-1-62703-511-8_6
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Tang, Yaoliang
中科院分区：
文献类型：
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作者：
Chen, Lijuan;Pan, Yaohua;Zhang, Lan;Wang, Yingjie;Weintraub, Neal;Tang, Yaoliang
心脏祖细胞是存在于心脏中的一组独特的祖细胞，在急性心肌梗死(MI)时心脏内稳态和生理性心血管细胞更新中发挥重要作用。在最近的两项心脏修复临床试验(Scipio和Caduceus)中，将CPC移植到心脏显示出了希望。干细胞最初是直接从酶消化的心脏中分离出来的，然后使用干细胞标记物进行细胞分选。然而，长期暴露在酶消化下会影响细胞表面干细胞标志物的完整性，也会损害干细胞的功能。在这里，我们描述了一个两步程序，在这个程序中，可以从少量的心脏组织中纯化大量完整的心脏前体细胞。
Cardiac progenitor cells (CPC) are a unique pool of progenitor cells residing in the heart that play an important role in cardiac homeostasis and physiological cardiovascular cell turnover during acute myocardial infarction (MI). Transplanting CPC into the heart has shown promise in two recent clinical trials of cardiac repair (SCIPIO & CADUCEUS). CSCs were originally isolated directly from enzymatically digested hearts followed by cell sorting using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface and also compromise stem cell function. Here, we describe a two-step procedure in which a large number of intact cardiac progenitor cells can be purified from small amount of heart tissue.