Acyclic retinoid inhibits functional interaction of transcription factors Kruppel-like factor 5 and retinoic acid receptor-alpha
Acyclic retinoid inhibits functional interaction of transcription factors Kruppel-like factor 5 and retinoic acid receptor-alpha
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DOI：
10.1016/j.febslet.2008.04.040
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发表时间：
2008-05-28
期刊：
影响因子：
3.5
通讯作者：
Nagai, Ryozo
中科院分区：
文献类型：
--
作者：
Kada, Nanae;Suzuki, Toru;Nagai, Ryozo
我们发现，转录因子Kruppel样因子5（KLF 5），这是重要的心血管重塑，与视黄酸受体α（RAR α）相互作用，调节下游基因的表达。在这里，我们研究了无环类维生素A（ACR）是否调节KLF 5和抑制血管重塑。免疫共沉淀和pull-down结合实验表明ACR减弱了KLF 5和RAR α的功能性相互作用。ACR通过调节血小板衍生生长因子A（PDGF-A）链的反式激活来影响KLF 5功能。ACR可能是一种新的血管治疗靶向KLF 5在心血管病理。(C)2008年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
We show that transcription factor Kruppel-like factor 5 (KLF5), which is important in cardiovascular remodeling, interacts with retinoic acid receptor-alpha (RAR alpha) to regulate downstream gene expression. Here, we investigated whether acyclic retinoid (ACR) regulates KLF5 and inhibits vascular remodeling. Co-immunoprecipitation and pull-down binding assay showed that ACR attenuates functional interaction of KLF5 and RAR alpha. ACR affects KLF5 functions by regulating transactivation of platelet-derived growth factor A (PDGF-A) chain. ACR may be a new vascular therapy to target KLF5 in cardiovascular pathology. (C) 2008 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.