Dynamic behavior of NK cells during activation in lymph nodes
Dynamic behavior of NK cells during activation in lymph nodes
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DOI：
10.1182/blood-2009-06-228759
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发表时间：
2009-10-08
期刊：
影响因子：
20.3
通讯作者：
Bousso, Philippe
中科院分区：
文献类型：
--
作者：
Beuneu, Helene;Deguine, Jacques;Bousso, Philippe
在感染过程中，Toll样受体激动剂通过刺激树突状细胞(DC)产生细胞因子并将IL-15转导至NK细胞来诱导自然杀伤(NK)细胞的激活。然而，次级淋巴器官中DC激活NK细胞的细胞动力学在很大程度上是未知的。在这里，我们用NK细胞和DC表达不同荧光蛋白的小鼠可视化了NK细胞的激活。作为对PolyI：C或脂多糖的响应，NK细胞保持了旺盛的迁移行为，与成熟的DC建立了多次短期接触。此外，成熟的抗原负载的DC与T细胞进行长期相互作用，与NK细胞形成短暂的接触。T细胞和NK细胞在活化过程中的不同行为与与DC相互作用时的不同钙反应有关。在次级淋巴组织中采集树突状细胞周围局部浓度的细胞因子时，NK细胞被激活而保持活动状态可能是一种有效的策略。(血。2009；114：3227-3234)
During infection, Toll-like receptor agonists induce natural killer (NK)-cell activation by stimulating dendritic cells (DCs) to produce cytokines and transpresent IL-15 to NK cells. Yet the cellular dynamics underlying NK-cell activation by DCs in secondary lymphoid organs are largely unknown. Here, we have visualized NK-cell activation using mice in which NK cells and DCs express different fluorescent proteins. In response to polyI:C or lipopolysaccharide, NK cells maintained a vigorous migratory behavior, establishing multiple short contacts with maturing DCs. Furthermore, mature antigen-loaded DCs that made long-lived interactions with T cells formed short-lived contacts with NK cells. The different behaviors of T cells and NK cells during activation was correlated with distinct calcium responses upon interaction with DCs. That NK cells become activated while remaining motile may constitute an efficient strategy for sampling local concentrations of cytokines around DCs in secondary lymphoid tissues. (Blood. 2009; 114: 3227-3234)