An analysis of the buffer systems in saliva
An analysis of the buffer systems in saliva
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DOI：
10.1177/00220345550340040701
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发表时间：
1955-01-01
期刊：
影响因子：
--
通讯作者：
LILIENTHAL, B.
中科院分区：
文献类型：
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作者：
LILIENTHAL, B.
在本研究中，采用了直接方法，首先通过用不含二氧化碳的空气去除碳酸氢盐，然后通过在 4℃ 下透析一起去除碳酸氢盐和磷酸盐来对唾液样品进行分级。研究发现，碳酸氢盐的去除显着降低了唾液的缓冲性能，表明碳酸氢盐系统是主要的唾液缓冲剂。去除碳酸氢盐和磷酸盐，留下粘蛋白和其他蛋白质，完全消除了唾液的缓冲能力。从唾液中分离出粘液，并通过直接滴定及其对几种 HCL 溶液 pH 值的影响进行检查，未能证实先前的报告，即唾液粘液具有相当大的缓冲特性。本研究中使用了静息唾液和活化唾液。观察到唾液沉积物和牙菌斑的缓冲系统存在差异，前者含有大量的碳酸氢盐。唾液样本中唾液沉积物的缓冲能力仅占样本总缓冲能力的很小一部分。 ||摘要作者：B. Lilienthal
In the present study a direct approach was used and salivary samples were fractionated firstly by removal of bicarbonate with CO2-free air and secondly by removal of bicarbonate and phosphate together by dialysis at 4[degree]C. It was found that removal of bicarbonate markedly reduced the buffering property of saliva, showing that the bicarbonate system was the major salivary buffer. Removal of both bicarbonate and phosphate, leaving mucin and other proteins, completely removed the buffering power of saliva. Mucoid isolated from saliva and examined by direct titration and by its effect on the pH of several solutions of HCL failed to confirm the previous report that salivary mucoid possessed considerable buffering properties. Both resting and activated saliva were used in this study. A difference in the buffer systems of salivary sediment and the dentobacterial plaque was observed, the former possessing appreciable quantities of bicarbonate. The buffering power of salivary sediment in a sample of saliva is a very small part of the total buffering of the sample. || ABSTRACT AUTHORS: B. Lilienthal