Demonstration of Tryptophan-Like Fluorescence Sensor Concepts for Fecal Exposure Detection in Drinking Water in Remote and Resource Constrained Settings
Demonstration of Tryptophan-Like Fluorescence Sensor Concepts for Fecal Exposure Detection in Drinking Water in Remote and Resource Constrained Settings
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DOI：
10.3390/su12093768
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发表时间：
2020-05-01
期刊：
影响因子：
3.9
通讯作者：
Thomas, Evan
中科院分区：
文献类型：
--
作者：
Bedell, Emily;Sharpe, Taylor;Thomas, Evan
在水传播感染风险最大的时间和地点，无法获得低成本、可现场部署、近时间的水质评估方法。我们描述了一种用于测量色氨酸样荧光（TLF）的新型装置的开发和测试，该装置利用了深紫外发光二极管（uv - led）和敏感半导体光电二极管和光电倍增管的最新进展。TLF是一种新兴的水质指标，与大肠菌群细菌成员有关，因此可能受到粪便污染。在证明了模型水体中TLF和大肠杆菌之间的密切相关性以及大肠杆菌灵敏度为4 CFU/mL的原理证明之后，我们进一步开发了一种双led流动配置，能够检测与“高风险”粪便污染水平（bbb10 CFU/100 mL）对应的TLF水平。迄今为止的研究结果表明，该设备代表了一种可扩展的解决方案，用于远程监测饮用水供应，以在近时间内识别高风险饮用水。这些信息可以立即采取行动，以减少风险。
Low-cost, field-deployable, near-time methods for assessing water quality are not available when and where waterborne infection risks are greatest. We describe the development and testing of a novel device for the measurement of tryptophan-like fluorescence (TLF), making use of recent advances in deep-ultraviolet light emitting diodes (UV-LEDs) and sensitive semiconductor photodiodes and photomultipliers. TLF is an emerging indicator of water quality that is associated with members of the coliform group of bacteria and therefore potential fecal contamination. Following the demonstration of close correlation between TLF and E. coli in model waters and proof of principle with sensitivity of 4 CFU/mL for E. coli, we further developed a two-LED flow-through configuration capable of detecting TLF levels corresponding to "high risk" fecal contamination levels (>10 CFU/100 mL). Findings to date suggest that this device represents a scalable solution for remote monitoring of drinking water supplies to identify high-risk drinking water in near-time. Such information can be immediately actionable to reduce risks.