Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants
Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants
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DOI：
10.1016/j.jep.2004.10.007
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发表时间：
2005-01-15
影响因子：
5.4
通讯作者：
Costa, SS
中科院分区：
文献类型：
--
作者：
Schmourlo, G;Mendonça-Filho, RR;Costa, SS
在寻找生物活性化合物的过程中，大分子（蛋白质、多糖等）的生物自显影和乙醇沉淀法，的植物水提取物进行了抗真菌筛选。因此，研究了巴西人群用于抗皮肤病的药用和果实植物的上清液、沉淀物（通过乙醇沉淀获得）和水提取物。采用琼脂扩散法和肉汤稀释法测定其对白色念珠菌、红色毛癣菌、金黄色葡萄球菌和金黄色葡萄球菌的抑菌活性。和新型隐球菌。结果表明，在16种植物中，有6种植物表现出明显的抗真菌活性。最小抑菌浓度（MIC）的植物提取物，显示出对测试的微生物的高效力确定。红色毛癣菌对该菌株的敏感性最高，箭叶黄体菌培养上清的抑菌活性最强。仅对对白色念珠菌和新型隐球菌显示抗真菌活性的那些植物的水提取物和上清液进行生物自显影，使用正丁醇/乙酸/水（BAW）8：1：1展开硅胶TLC板。对白念珠菌的抑菌圈清晰可见，翅果木（R-f0. 89）和黑木（R-f0. 80）的水提物对白念珠菌的抑菌圈清晰可见，而对新生隐球菌的生长抑制作用则明显可见。从代谢物中分离大分子，如在腰果、茄属和箭叶黄虫的情况下，增强抗真菌活性。在其他情况下，抗真菌活性被破坏，如观察到的Momorea charantia，Schinus molle和Schinus terebinthifolius。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
In the search for bioactive compounds, bioautography and ethanol precipitation of macromolecules (proteins, polysaccharides, etc.) of plant aqueous extracts were associated in an antifungal screening. Thus, the supernatants, precipitates (obtained by ethanol precipitation) and aqueous extracts were investigated of medicinal and fruit bearing plants used against skin diseases by the Brazilian population. The agar diffusion and broth dilution methods were used to assess the activity against three fungi: Candida albicans, Trichophyton rubrum? and Cryptococcus neoformans. The results, evaluated by the diameter of the inhibition zone of fungal growth, indicate that six plant species, among the 16 investigated, showed significant antifungal activity. The minimal inhibitory concentration (MIC) was determined on plant extracts that showed high efficacy against the tested microorganisms. The most susceptible yeast was Trichophyton rubrum and the best antifungal activity was shown by Xanthosoma sagittifolium supernatant. The bioautography was performed only for the aqueous extracts and supernatants of those plants that showed antifungal activity against Candida albicans and Cryptococcus neoformans, using n-butanol/acetic acid/water (BAW) 8: 1:1 to develop silica gel TLC plates. Clear inhibition zones were observed for aqueous extracts of Schinus molle (R-f 0.89) and Schinus terebinthifolius (R-f 0.80) against Candida albicans, as for supernatant of Anacardium occidentale (R-f 0.31) against Cryptococcus neoformans. The separation of macromolecules from metabolites, as in the case of Anacardium occidentale, Solanum sp. and Xanthosoma sagittifolium, enhances antifungal activity. In other cases, the antifungal activity is destroyed, as observed for Momordica charantia, Schinus molle and Schinus terebinthifolius. (C) 2004 Elsevier Ireland Ltd. All rights reserved.