Upregulation of monocyte urokinase plasminogen activator receptor during human endotoxemia
Upregulation of monocyte urokinase plasminogen activator receptor during human endotoxemia
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DOI：
10.1128/iai.68.4.2156-2160.2000
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发表时间：
2000-04-01
影响因子：
3.1
通讯作者：
Van der Poll, T
中科院分区：
文献类型：
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作者：
Dekkers, PEP;Ten Hove, T;Van der Poll, T
尿激酶型纤溶酶原激活物（uPAR）受体（CD 87）在白细胞粘附和迁移中起重要作用。为了评估内毒素对细胞uPAR的影响，在静脉内注射内毒素（IOtG; 4 ng/kg）后，在7名健康受试者的白细胞bg荧光激活细胞分选仪分析上测定uPAR表达。内毒素诱导单核细胞上uPAR表达的瞬时增加，在6小时后达到超过基线表达的92% +/-46%的增加（P < 0.05）。内毒素没有影响uPAR表达的粒细胞，而uPAR仍然检测不到淋巴细胞。血浆可溶性uPAR水平亦显著升高（P < 0.05）。在体外用内毒素或革兰氏阳性刺激物刺激人全血也导致单核细胞uPAR表达上调。虽然肿瘤坏死因子α（TNF）上调单核细胞uPAR表达，抗TNF并不影响内毒素诱导的单核细胞uPAR表达的增加。这些数据表明，感染性刺激可能会影响单核细胞功能，在体内通过增强uPAR的表达。
The receptor for urokinase-type plasminogen activator (uPAR) (CD87) plays an important role in leukocyte adhesion and migration. To assess the effect of endotoxin on cellular uPAR, uPAR expression was determined on leukocytes bg fluorescence-activated cell sorter analysis in seven healthy subjects following intravenous injection of endotoxin (Iot G; 4 ng/kg). Endotoxin induced a transient increase in uPAR expression on monocytes, reaching a 92% +/- 46% increase over baseline expression after 6 h (P < 0.05). Endotoxin did not influence uPAR expression on granulocytes, while uPAR remained undetectable on lymphocytes. Endotoxin also increased soluble uPAR levels in plasma (P < 0.05). Stimulation of human whole blood with endotoxin or gram-positive stimuli in vitro also resulted in an upregulation of monocyte uPAR expression. Although tumor necrosis factor alpha (TNF) upregulated monocyte uPAR expression, anti-TNF did not influence the endotoxin-induced increase in monocyte uPAR expression. These data suggest that infectious stimuli may influence monocyte function in vivo by enhancing the expression of uPAR.