Discovery of highly specific protein markers for the identification of biological stains
Discovery of highly specific protein markers for the identification of biological stains
复制标题
DOI：
10.1002/elps.201400125
复制
发表时间：
2014-11-01
期刊：
影响因子：
2.9
通讯作者：
Danielson, Phillip B.
中科院分区：
文献类型：
--
作者：
Legg, Kevin M.;Powell, Roger;Danielson, Phillip B.
DNA图谱分析使生物染色的个体化成为可能，从而改变了法医生物学领域。然而，对染色剂本身的鉴定仍然给法医血清学家带来重大挑战。目前基于抗体和酶活性的检测仅产生推定结果，因为在非靶体液中的检测或与非人类来源的交叉反应性都已得到充分证明。对于其他重要的体液，如阴道液和月经液，根本没有商业测试。使用三管齐下，比较蛋白质组学策略的基础上，蛋白质组分离的HPLC，然后MS，一组29个候选蛋白质生物标志物已被提出作为高度特异性指标的人唾液，尿液，精液，阴道液，外周血，和月经液。在当前研究中通过多种策略进行一致鉴定的组合;在独立编译的蛋白质组学数据库中的确认;以及来自蛋白质组学文献的关于组织表达和/或功能的信息都支持这些蛋白质将作为其靶体液的可靠生物标志物的实用性的主张。鉴定法医学调查中遇到的人体体液的候选高特异性蛋白质生物标志物为发展更快，更可靠的方法来对证据污渍进行血清学分析奠定了基础。
DNA profiling has transformed the field of forensic biology by making it possible to individualize biological stains. The identification of the stain itself, however, continues to present forensic serologists with significant challenges. Current antibody-and enzyme activity-based assays yield only presumptive results as detection in nontarget body fluids or cross-reactivity with nonhuman sources have both been well documented. For other critical body fluids such as vaginal and menstrual fluids, there are no commercial tests at all. Using a three-pronged, comparative proteomic strategy based on proteome fractionation by HPLC followed by MS, a panel of 29 candidate protein biomarkers have been proposed as highly specific indicators of human saliva, urine, seminal fluid, vaginal fluid, peripheral blood, and menstrual fluid. The combination of consistent identification by multiple strategies in the current study; confirmation in independently compiled proteomic databases; and information on tissue expression and/or functionality from the proteomic literature all support the proposition that these proteins will have utility as reliable biomarkers of their target body fluids. The identification of candidate high-specificity protein biomarkers for human body fluids encountered in forensic investigations lays the foundation for the development of faster and more reliable approaches to the serological analysis of evidentiary stains.