Retroviral E-DNA: persistence and gene expression in nondividing immune cells
Retroviral E-DNA: persistence and gene expression in nondividing immune cells
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DOI：
10.1189/jlb.0306151
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发表时间：
2006-11-01
影响因子：
5.5
通讯作者：
Klotman, Mary E.
中科院分区：
文献类型：
--
作者：
Cara, Andrea;Klotman, Mary E.
在逆转录病毒感染细胞后，不仅前病毒DNA整合到宿主基因组中，而且线性和环状的未整合的染色体外DNA（E-DNA）也积累。虽然整合的DNA负责病毒蛋白质和新病毒后代的产生，但E-DNA的作用仍然不确定。一些报告表明，E-DNA是转录活性产生RNA，以及病毒蛋白质和环状E-DNA可以坚持在非分裂细胞，提出了关于这个水库的潜在后果的问题。此外，目前在临床试验中的整合酶抑制剂将前病毒DNA的平衡转移到E-DNA形式。本文综述了E-DNA在逆转录病毒感染，特别是HIV-1的发病机制中的潜在作用，以及E-DNA作为传递和表达基因的工具。J. Leukoc. 80：1013-1017; 2006.
Following retroviral infection of cells, not only is the proviral DNA integrated into the host genome, but there is also an accumulation of unintegrated extrachromosomal DNA (E-DNA), both linear and circular. Although the integrated DNA is responsible for the production of viral proteins and new viral progeny, the role of E-DNA has remained uncertain. Several reports have shown that E-DNA is transcriptionally active producing both RNA, as well as viral proteins and that circular E-DNA can persist in nondividing cells, raising questions regarding the potential consequences of this reservoir. Furthermore, integrase inhibitors, presently in clinical trials, shifts the balance of proviral DNA to the E-DNA form. This review is focused on recent work in this field with an emphasis on exploring the potential role of E-DNA in both pathogenesis of retroviral infections, especially HIV-1, and as a tool to deliver and express genes. J. Leukoc. Biol. 80: 1013-1017; 2006.