Circulation of progenitor cells in obese and lean colorectal cancer patients.
Circulation of progenitor cells in obese and lean colorectal cancer patients.
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DOI：
10.1158/1055-9965.epi-11-0556
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Kolonin MG
中科院分区：
文献类型：
--
作者：
Bellows CF;Zhang Y;Chen J;Frazier ML;Kolonin MG
结直肠癌（CRC）是一种常见的威胁生命的恶性肿瘤，而肥胖症的目的是衡量循环CD34阳性内皮细胞的频率，并评估其潜在值作为CRC生物标志物。
从45名CRC患者中收集血液，并与无癌的对照供体进行比较。 （EC），间质基质细胞（MSC）和CD34Bright白细胞（CD34B LC）使用多元回归分析进行比较。接收器操作特征（ROC）曲线分析用于评估诊断值。
调整年龄和BMI后，与对照组相比，CRC患者的循环中MSC和CD34BLC的平均频率分别为MSC和CD34B LC的0.82。 ，但与瘦肉相比CRC患者和肥胖对照。
外周血中MSC和CD34B LC的频率增加可能代表CRC的新诊断标记。
循环MSC的BMI依赖性变化，可能是由白脂肪组织动员的，可能会揭示其贩运对肿瘤的运输，这可能是肥胖与癌症进展之间的机械联系之一。
Colorectal cancer (CRC) is a common life-threatening malignancy; risk and progression are elevated in obesity. The purpose of this study was to measure the frequency of circulating CD34 positive endothelial and progenitor cells in the circulation, and evaluate their potential values as CRC biomarkers. Blood was collected from 45 CRC patients and compared to cancer-free control donors. Detection and enumeration of cells was performed by using flow cytometry based on immunophenotypes established for the cell populations of interest: hematopoietic and endothelial circulating progenitor cells (CPC), endothelial cells (EC), mesenchymal stromal cells (MSC) and CD34bright leukocytes (CD34b LC). Groups were compared using multivariate regression analysis. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic values. After adjusting for age and BMI, the mean frequencies of MSC and CD34bLC were significantly higher in the circulation of CRC patients compared to controls. The areas under the ROC curve were 0.77 and 0.82 for MSC and CD34b LC, respectively. The frequency of circulating MSC, but not of the other cell populations, was also found to be significantly higher in the circulation of obese CRC patients (BMI ≥ 30 kg/m2) compared to lean CRC patients and obese controls. Increased frequency of MSC and CD34b LC in the peripheral blood may represent a new diagnostic marker for CRC. BMI-dependent changes in circulating MSC, potentially mobilized from white adipose tissue may reveal their trafficking to tumors, which could be one of the mechanistic links between obesity and cancer progression.