Functional CT for quantifying tumor perfusion in antiangiogenic therapy in a rat model
Functional CT for quantifying tumor perfusion in antiangiogenic therapy in a rat model
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DOI：
10.1148/radiol.2363041293
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发表时间：
2005-10-01
期刊：
影响因子：
19.7
通讯作者：
Charnsangavej, C
中科院分区：
文献类型：
--
作者：
Kan, ZX;Phongkitkarun, S;Charnsangavej, C
目得：为了确定在功能性计算机断层扫描（CT）测量的灌注参数的组织学基础，并检查灌注的变化和组织学参数的变化后，在大鼠model.MATERIALS和METHODS：本研究机构的动物护理和使用委员会的批准之间的关系。在20只肝脏植入FN 13762肿瘤的Fischer大鼠中，10只用血管内皮生长因子受体酪氨酸激酶抑制剂SU 5416治疗，10只仅用稀释剂治疗作为对照大鼠。从每组中随机选择6只h大鼠进行功能CT，测量肿瘤血流量、血容量、平均通过时间和渗透性表面积乘积。检查对应于功能CT切片的肿瘤组织载玻片，以测量肿瘤微血管密度、管腔血管数量、血管周长和血管面积。双尾Student t检验用于确定SU 5416治疗和对照肿瘤之间的生长、主要器官转移数量、血管分布和灌注差异。结果：SU 5416处理组大鼠的平均肿瘤体积和转移灶数目均低于对照组大鼠（1580 mm（3）+/- 830 [标准差] vs 2330 mm（3）+/- 960和22.4 +/- 11.0 vs 35.2 +/- 17.3）;然而，这些差异不显著（P = 0.084和P = 0.079）。SU 5416治疗组大鼠的平均肿瘤微血管密度显著低于对照组大鼠（6.4个血管/视野+/- 4.6 vs 17.2个血管/视野+/- 7.5，P
PURPOSE: To determine the histologic basis of perfusion parameters measured at functional computed tomography (CT) and to examine the relationship between changes in perfusion and changes in histologic parameters after antiangiogenic therapy in a rat model.MATERIALS AND METHODS: This study had institutional animal care and use committee approval. Among 20 Fischer rats with implanted FN13762 tumors in the liver, 10 were treated with SU5416, a tyrosine kinase inhibitor of vascular endothelial growth factor receptor, and 10 were treated with the diluent only as control rats. Six h rats chosen at random from each group underwent functional CT for the measurement of tumor blood flow, blood volume, mean transit time, and permeability-surface area product. Tumor tissue slides corresponding to functional CT sections were examined to measure tumor microvascular density, number of luminal vessels, vascular perimeter, and vascular area. Two-tailed Student t testing was used to determine differences in growth, numbers of metastases to major organs, vascularity, and perfusion between SU5416-treated and control tumors. Pearson correlation coefficients were used to investigate relationships between vascular parameters.RESULTS: Mean tumor volume and number of metastases, respectively, were lower in SU5416-treated rats than in control rats (1580 mm(3) +/- 830 [standard deviation] vs 2330 mm(3) +/- 960 and 22.4 +/- 11.0 vs 35.2 +/- 17.3); however, these differences were not significant (P =.084 and P =.079). Mean tumor microvascular density was significantly lower in SU5416-treated rats than in control rats (6.4 vessels per field +/- 4.6 vs 17.2 vessels per field +/- 7.5, P