BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.
BmILF and i-motif structure are involved in transcriptional regulation of BmPOUM2 in Bombyx mori.
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BmILF 和 i-motif 结构参与家蚕 BmPOUM2 的转录调控
DOI：
10.1093/nar/gkx1207
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发表时间：
2018-02-28
影响因子：
14.9
通讯作者：
Feng Q
中科院分区：
文献类型：
--
作者：
Niu K;Zhang X;Deng H;Wu F;Ren Y;Xiang H;Zheng S;Liu L;Huang L;Zeng B;Li S;Xia Q;Song Q;Palli SR;Feng Q
富含鸟嘌呤和胞嘧啶的DNA可形成四链DNA二级结构，分别称为G-四链体（G4）和i-基序。这些结构广泛存在于基因组中，在端粒的转录、复制、翻译和保护中起着重要作用。在这项研究中，G4和i-motif结构被确定在转录因子基因BmPOUM 2的启动子中，该基因在变态期间调节翅盘角质层蛋白基因（BmWCP 4）的表达。通过碱基突变、反义寡核苷酸（ASO）或抑制性配体破坏i基序结构导致BmPOUM 2启动子的活性显著降低。通过pull-down实验鉴定了一种新的i-motif结合蛋白（BmILF）。BmILF特异性地结合到i基序并激活BmPOUM 2的转录。BmPOUM 2的启动子活性在BmILF过表达时增强，在BmILF被RNA干扰敲低时降低。本研究首次证实了BmILF和i-motif结构参与了昆虫变态过程中基因转录的调控，为进一步了解二级结构在基因转录表观遗传调控中的分子机制提供了新的思路。
Abstract Guanine-rich and cytosine-rich DNA can form four-stranded DNA secondary structures called G-quadruplex (G4) and i-motif, respectively. These structures widely exist in genomes and play important roles in transcription, replication, translation and protection of telomeres. In this study, G4 and i-motif structures were identified in the promoter of the transcription factor gene BmPOUM2, which regulates the expression of the wing disc cuticle protein gene (BmWCP4) during metamorphosis. Disruption of the i-motif structure by base mutation, anti-sense oligonucleotides (ASOs) or inhibitory ligands resulted in significant decrease in the activity of the BmPOUM2 promoter. A novel i-motif binding protein (BmILF) was identified by pull-down experiment. BmILF specifically bound to the i-motif and activated the transcription of BmPOUM2. The promoter activity of BmPOUM2 was enhanced when BmILF was over-expressed and decreased when BmILF was knocked-down by RNA interference. This study for the first time demonstrated that BmILF and the i-motif structure participated in the regulation of gene transcription in insect metamorphosis and provides new insights into the molecular mechanism of the secondary structures in epigenetic regulation of gene transcription.
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影响因子：
16.6
作者：
Banerjee, Kasturi;Wang, Meng;Cai, Elizabeth;Fujiwara, Nana;Baker, Harriet;Cave, John W.
通讯作者：
Cave, John W.
影响因子：
46.9
作者：
Chambers, Vicki S.;Marsico, Giovanni;Balasubramanian, Shankar
通讯作者：
Balasubramanian, Shankar
影响因子：
15
作者：
Dingley, AJ;Peterson, RD;Feigon, J
通讯作者：
Feigon, J
影响因子：
14.9
作者：
Guo K;Gokhale V;Hurley LH;Sun D
通讯作者：
Sun D
影响因子：
2.9
作者：
Fedoroff, OY;Rangan, A;Hurley, LH
通讯作者：
Hurley, LH