[Expression and subcellular localization of APOBEC3G in peripheral blood mononuclear cells and liver tissues of chronic HBV patients].
[Expression and subcellular localization of APOBEC3G in peripheral blood mononuclear cells and liver tissues of chronic HBV patients].
复制标题
DOI：
10.3760/cma.j.issn.1007-3418.2010.01.002
复制
发表时间：
2010-01-01
影响因子：
--
通讯作者：
Gong, Zuo-Jiong
中科院分区：
文献类型：
--
作者：
Chen, Hui;Wang, Lu-Wen;Gong, Zuo-Jiong
目的：目的：研究慢性乙型肝炎患者外周血单个核细胞（PBMCs）和肝组织中APOBEC 3G的表达水平和细胞内定位。方法：采用Western blot和激光共聚焦扫描显微镜（CLSM）检测慢性乙型肝炎患者PBMCs和肝组织中APOBEC 3G的表达水平和细胞内定位。Western-blot结果显示，健康对照组PBMC中APOBEC 3G的表达水平很低。慢性B型肝炎、慢性重型肝炎、肝硬化和肝癌患者PBMC中APOBEC 3G的相对表达水平分别为4.12 ± 0.21、4.07 ± 0.28、4.16 ± 0.36和4.21 ± 0.39，均高于健康对照组。而APOBEC 3G在不同慢性HBV患者中的表达差异无统计学意义（q值分别为0.931、0.744、1.675、1.675、2.606和0.931，P值均大于0.05）。慢性B型肝炎患者肝组织中APOBEC 3G的表达与肝癌患者肝组织中APOBEC 3G的表达无显著性差异（4.40 ± 0.34 vs 4.34 ± 0.43，q = 0.588，P > 0.05）。结论：慢性B型肝炎患者外周血单个核细胞中APOBEC 3G表达上调。
OBJECTIVE: To study the expression level and intracellular localization of APOBEC3G in peripheral blood mononuclear cells (PBMCs) and liver tissues of chronic HBV patients.METHODS: The expression level and intracellular localization of APOBEC3G in PBMCs and liver tissues were detected using the western blot and confocal laser scanning microscope (CLSM).RESULTS: Western-blot showed that the expression level of APOBEC3G in PBMCs of healthy controls was very low. The relative expression levels of APOBEC3G in PBMC of patients with chronic hepatitis B, chronic severe hepatitis, liver cirrhosis, or liver cancer were 4.12+/-0.21, 4.07+/-0.28, 4.16+/-0.36 or 4.21+/-0.39 respectively, which were higher than that in the healthy controls. However, there was no significant difference in APOBEC3G expression among different chronic HBV patients (q = 0.931, 0.744, 1.675, 1.675, 2.606 or 0.931, respectively, all P values more than 0.05). In addition, there was no significant difference on APOBEC3G in liver tissues between chronic hepatitis B patients and hepatocellular carcinoma patients (4.40+/-0.34 vs 4.34+/-0.43, q = 0.588, P more than 0.05). CLSM indicated that the localization of APOBEC3G protein was in cytoplasm of PBMCs and hepatocytes.CONCLUSION: APOBEC3G is upregulated in the PBMCs of chronic hepatitis B patients.