Re-emergence of amantadine-resistant variants among highly pathogenic avian influenza H5N1 viruses in Egypt
Re-emergence of amantadine-resistant variants among highly pathogenic avian influenza H5N1 viruses in Egypt
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DOI：
10.1016/j.meegid.2016.10.022
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发表时间：
2016-12-01
影响因子：
3.2
通讯作者：
Kayali, Ghazi
中科院分区：
文献类型：
--
作者：
El-Shesheny, Rabeh;Bagato, Ola;Kayali, Ghazi
高致病性禽流感（HPAI）H5 N1病毒继续经历实质性演变。H5 N1禽流感病毒抗病毒耐药性的出现是控制大流行性流感的一个重大挑战。大量的研究集中在血凝素和神经氨酸酶基因的遗传和进化动力学;然而，关于HPAI H5 N1病毒对金刚烷胺的易感性和基质（M）基因的遗传多样性的研究有限。因此，我们基于基因型和表型特征研究了2006-2015年期间在埃及分离的HPAI H5 N1病毒的金刚烷胺敏感性。我们分析了253个病毒序列的数据，并构建了一个系统发育树，以计算不同进化枝之间与金刚烷胺耐药性相关的M2基因位点的选择压力。在M2基因的跨膜结构域的位置27和31处确定了选择压力。耐金刚烷胺的变种于2007年出现，但在2012年至2014年期间没有传播。到2015年，耐金刚烷胺的HPAI H5 N1病毒再次出现。这可能与埃及家禽养殖部门为预防和控制禽流感感染而不加控制地开具金刚烷胺处方有关。需要更多的流行病学研究来验证这一观察结果。(C)2016爱思唯尔B. V.保留所有权利。
Highly pathogenic avian influenza (HPAI) H5N1 virus continues to undergo substantial evolution. Emergence of antiviral resistance among H5N1 avian influenza viruses is amajor challenge in the control of pandemic influenza. Numerous studies have focused on the genetic and evolutionary dynamics of the hemagglutinin and neuraminidase genes; however, studies on the susceptibility of HPAI H5N1 viruses to amantadine and genetic diversity of the matrix (M) gene are limited. Accordingly, we studied the amantadine susceptibility of the HPAI H5N1 viruses isolated in Egypt during 2006-2015 based on genotypic and phenotypic characteristics. We analyzed data on 253 virus sequences and constructed a phylogenetic tree to calculate selective pressures on sites in the M2 gene associated with amantadine-resistance among different clades. Selection pressure was identified in the transmembrane domain of M2 gene at positions 27 and 31. Amantadine-resistant variants emerged in 2007 but were not circulating between 2012 and 2014. By 2015, amantadine-resistant HPAI H5N1 viruses re-emerged. This may be associated with the uncontrolled prescription of amantadine for prophylaxis and control of avian influenza infections in the poultry farmsector in Egypt. More epidemiological research is required to verify this observation. (C) 2016 Elsevier B.V. All rights reserved.