Diversity of immunoglobulin expression in leukaemic cells resembling B-lymphocyte precursors
Diversity of immunoglobulin expression in leukaemic cells resembling B-lymphocyte precursors
复制标题
类似于 B 淋巴细胞前体的白血病细胞中免疫球蛋白表达的多样性
作者：
L. Vogler;J. Preud’homme;M. Seligmann;W. Gathings;W. Crist;M. Cooper;F. Bollum
大约20%的急性淋巴细胞白血病（ALL）患者具有前B细胞特征的白血病原细胞1，这是最近发现的正常发育的B淋巴细胞的前体（回顾，见参考文献2）。从正常骨髓或胎儿肝脏中分离的前b细胞，以及从前b细胞白血病患者中分离的恶性细胞，是快速分裂的淋巴样细胞，含有细胞质免疫球蛋白μ重链，但没有检测到表面免疫球蛋白。含有免疫球蛋白的ALL细胞与B淋巴细胞的正常前体的相似性及其在相对纯净的制剂中的可用性使我们能够探索它们作为B淋巴细胞系分化早期阶段的模型。我们在此报告了30例ALL和3例慢性髓性白血病淋巴细胞危象（CML-BC）中出现的中间前b / b细胞表型、免疫球蛋白同型转换和免疫球蛋白重链和轻链表达的不同步。
Approximately 20% of patients with acute lymphocytic leukaemia (ALL) have leukaemic blasts with features of pre-B cells1 which are the recently characterized precursors of B lymphocytes in normal development (for a review, see ref. 2). Pre-B cells isolated from normal bone marrow or fetal liver, and malignant cells from patients with pre-B cell leukaemia, are rapidly dividing lymphoid cells that contain cytoplasmic immunoglobulin μ heavy chains, but have no detectable surface immunoglobulin. The resemblance of immunoglobulin-containing ALL cells to normal precursors of B lymphocytes and their availability in relatively pure preparations allowed us to explore them as models of early stages in the differentiation of the B-lymphocyte line. We report here observations on the occurrence of intermediate pre-B/B-cell phenotypes, immunoglobulin isotype switching and the asynchrony of immunoglobulin heavy and light chain expression in 30 cases of ALL and 3 cases of chronic myelogenous leukaemia in lymphoblastic crisis (CML-BC).