Complementation of a red-light-indifferent cyanobacterial mutant.
Complementation of a red-light-indifferent cyanobacterial mutant.
复制标题
红光无关的蓝藻突变体的互补。
DOI：
10.1073/pnas.89.20.9415
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发表时间：
1992
影响因子：
11.1
通讯作者：
Grossman,AR
中科院分区：
文献类型：
--
作者：
Chiang,GG;Schaefer,MR;Grossman,AR
许多蓝细菌改变其藻胆体组成，以响应光波长的变化，在一个过程中被称为互补色适应。丝状蓝藻Fremyella diplosiphon的突变株FdR 1和FdR 2的特征在于异常的色适应。FdR 1和FdR 2并没有适应不同波长的光，而是表现得好像它们总是处于绿色光中;它们对红光没有反应。我们以前已经报道了互补的FdR 1的野生型基因组文库的接合转移。通过遗传分析，互补DNA现在已经定位到拯救的基因组亚克隆上的一个区域，该区域包含一个名为rcaC的基因。该DNA区域也能够与FdR 2互补。FdR 1和FdR 2基因组DNA的Southern印迹分析表明，这些菌株的rcaC序列内可能有转座遗传因子的活性导致的DNA插入。预测的RcaC的氨基酸序列与细菌双组分调节系统的反应调节剂具有很强的同一性。这种关系进行了讨论的背景下的信号转导途径介导的调控基因编码的藻胆体多肽在色适应。
Many cyanobacteria alter their phycobilisome composition in response to changes in light wavelength in a process termed complementary chromatic adaptation. Mutant strains FdR1 and FdR2 of the filamentous cyanobacterium Fremyella diplosiphon are characterized by aberrant chromatic adaptation. Instead of adjusting to different wavelengths of light, FdR1 and FdR2 behave as if they are always in green light; they do not respond to red light. We have previously reported complementation of FdR1 by conjugal transfer of a wild-type genomic library. The complementing DNA has now been localized by genetic analysis to a region on the rescued genomic subclone that contains a gene designated rcaC. This region of DNA is also able to complement FdR2. Southern blot analysis of genomic DNA from FdR1 and FdR2 indicates that these strains harbor DNA insertions within the rcaC sequence that may have resulted from the activity of transposable genetic elements. The predicted amino acid sequence of RcaC shares strong identity to response regulators of bacterial two-component regulatory systems. This relationship is discussed in the context of the signal-transduction pathway mediating regulation of genes encoding phycobilisome polypeptides during chromatic adaptation.