Differentiation between control subjects and patients with chronic spontaneous urticaria based on the ability of anti-IgE autoantibodies (AAbs) to induce FcεRI crosslinking, as compared to anti-FcεRIα AAbs
Differentiation between control subjects and patients with chronic spontaneous urticaria based on the ability of anti-IgE autoantibodies (AAbs) to induce FcεRI crosslinking, as compared to anti-FcεRIα AAbs
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DOI：
10.1016/j.alit.2019.01.003
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发表时间：
2019-07-01
影响因子：
6.8
通讯作者：
Okayama, Yoshimichi
中科院分区：
文献类型：
--
作者：
Izaki, Satoshi;Toyoshima, Shota;Okayama, Yoshimichi
背景资料：据报道，慢性自发性荨麻疹（CSU）患者血清中Fc β RI α和IgE的IgG自身抗体（AAb）的患病率各不相同，这些AAb也经常在健康对照受试者中观察到。关于从CSU患者纯化的IgG的组胺释放活性，检查的患者数量很少。因此，我们试图确定这些AAb在大量CSU患者和非特应性对照（NC）受试者中的患病率和Fc ε RI交联能力。我们比较了CSU患者（n = 134）和NC受试者（n = 55）中抗IgE和抗Fc β RI α AAb的浓度以及这些AAb引起Fc β RI聚集的能力。结果：正常对照组和慢性SU组患者血清中抗IgE AAbs的浓度有显著性差异（P < 0.0001，临界值为0.558 μ g/mL），而抗Fc γ RI α AAbs的浓度无显著性差异。相对于临界值，在具有较低和较高浓度抗IgE AAb的患者之间注意到疾病持续时间的显著差异。CSU患者中抗IgE AAbs诱导Fc ε RI交联的能力显著高于NC受试者，但抗Fc ε RI α AAbs诱导Fc ε RI交联的能力不显著（P = 0.0106）。在研究的日本CSU患者人群中，抗IgE AAb诱导Fc ε RI交联的能力在NC受试者和CSU患者之间显著不同，提示日本人群中CSU的发病机制与抗IgE AAb有关。Copyright（C）2019，日本变态反应学会制作和主办：Elsevier B. V.
Background: The reported prevalences of IgG autoantibodies (AAbs) to Fc epsilon RI alpha and IgE in sera from patients with chronic spontaneous urticaria (CSU) have varied, and these AAbs are also often observed in healthy control subjects. Regarding the histamine release activity of purified IgG from patients with CSU, the number of examined patients has been small. Thus, we sought to determine the prevalence and Fc epsilon RI crosslinking ability of these AAbs in a large number of patients with CSU and non-atopic control (NC) subjects.Methods: We compared the concentrations of anti-IgE and anti-Fc epsilon RI alpha AAbs and the abilities of these AAbs to cause Fc epsilon RI aggregation in patients with CSU (n = 134) and NC subjects (n = 55) using ELISA and an in vitro elicitation test, respectively.Results: The concentration of anti-IgE AAbs was significantly different between the NC subjects and the CSU patients (P < 0.0001, cutoff value: 0.558 mu g/mL), whereas the concentration of anti-Fc epsilon RI alpha AAbs was not. A significant difference in the duration of illness was noted between patients with lower and those with higher concentrations of anti-IgE AAbs relative to the cutoff value. The abilities of anti-IgE AAbs, but not anti-Fc epsilon RI alpha AAbs, to induce Fc epsilon RI crosslinking were significantly higher in CSU patients than in NC subjects (P = 0.0106).Conclusions: In the Japanese population of CSU patients studied, the ability of the anti-IgE AAbs to induce Fc epsilon RI crosslinking differed significantly between NC subjects and CSU patients, suggesting the involvement of anti-IgE AAbs in the pathogenesis of CSU in the Japanese population. Copyright (C) 2019, Japanese Society of Allergology. Production and hosting by Elsevier B.V.