Xanthomonas translucens commandeers the host rate-limiting step in ABA biosynthesis for disease susceptibility
Xanthomonas translucens commandeers the host rate-limiting step in ABA biosynthesis for disease susceptibility
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DOI：
10.1073/pnas.1911660116
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发表时间：
2019-10-15
影响因子：
11.1
通讯作者：
White, Frank F.
中科院分区：
文献类型：
--
作者：
Peng, Zhao;Hu, Ying;White, Frank F.
由于病原体对植物激素水平的操纵，植物容易受到疾病的影响，而植物激素水平则调节发育、非生物和生物反应。在这里，我们展示了小麦病原体 Xanthomonas translucens pv。 undulosa 提高编码 9-顺式环氧类胡萝卜素双加氧酶 (TaNCED-5BS) 的宿主基因的表达，该酶催化植物激素脱落酸生物合成中的限速步骤，也是主要非生物应激反应途径的一个组成部分，从而促进疾病易感性。基因诱导由III型转录激活子样效应子介导。 TaNCED-5BS 的诱导导致脱落酸水平升高、宿主蒸腾作用和水分流失减少、受感染叶子中细菌的传播增强以及中枢防御基因 TaNPR1 的表达降低。结果代表细菌毒力效应器对宿主生理学的占用。
Plants are vulnerable to disease through pathogen manipulation of phytohormone levels, which otherwise regulate development, abiotic, and biotic responses. Here, we show that the wheat pathogen Xanthomonas translucens pv. undulosa elevates expression of the host gene encoding 9-cis-epoxycarotenoid dioxygenase (TaNCED-5BS), which catalyzes the rate-limiting step in the biosynthesis of the phytohormone abscisic acid and a component of a major abiotic stress-response pathway, to promote disease susceptibility. Gene induction is mediated by a type Ill transcription activator-like effector. The induction of TaNCED-5BS results in elevated abscisic acid levels, reduced host transpiration and water loss, enhanced spread of bacteria in infected leaves, and decreased expression of the central defense gene TaNPR1. The results represent an appropriation of host physiology by a bacterial virulence effector.