DNA Triplexes-Guided Assembly of G-Quadruplexes for Constructing Label-free Fluorescent Logic Gates
DNA Triplexes-Guided Assembly of G-Quadruplexes for Constructing Label-free Fluorescent Logic Gates
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DNA 三链体引导 G-四链体组装，用于构建无标记荧光逻辑门
DOI：
10.1002/asia.201600626
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发表时间：
2016
影响因子：
4.1
通讯作者：
Pei Renjun
中科院分区：
文献类型：
--
作者：
Xu Lijun;Hong Shanni;Shen Xiaoqiang;Zhou Lu;Wang Jine;Zhang Jianye;Pei Renjun
首次实现了 DNA 三链体引导下 G-四链体以受控方式的组装。在酸性条件下，三链体序列的折叠将两个分离的富含鸟嘌呤的序列结合在一起，随后在 K+ 存在的情况下形成 G-四链体结构。基于这个新颖的平台，构建了无标记荧光逻辑门，例如 AND、INHIBIT 和 NOR，以离子作为输入，G-四链体特异性荧光探针 NMM 的荧光作为输出。
Assembly of G‐quadruplexes guided by DNA triplexes in a controlled manner is achieved for the first time. The folding of triplex sequences in acidic conditions brings two separated guanine‐rich sequences together and subsequently a G‐quadruplex structure is formed in the presence of K+. Based on this novel platform, label‐free fluorescent logic gates, such as AND, INHIBIT, and NOR, are constructed with ions as input and the fluorescence of a G‐quadruplex‐specific fluorescent probe NMM as output.