One step screening of retroviral producer clones by real time quantitative PCR
One step screening of retroviral producer clones by real time quantitative PCR
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通过实时定量 PCR 一步筛选逆转录病毒生产克隆
作者：
G. Towers;D. Stockholm;Valérie Labrousse‐Najburg;F. Carlier;O. Danos;Jean
重组逆转录病毒获自稳定或瞬时转染的逆转录病毒生产细胞。在稳定生产的品系的情况下，必须筛选大量的克隆以选择具有最高滴度的克隆。高滴度生产克隆的多步选择耗时且昂贵。
Recombinant retroviruses are obtained from either stably or transiently transfected retrovirus producer cells. In the case of stably producing lines, a large number of clones must be screened in order to select the one with the highest titre. The multi‐step selection of high titre producing clones is time consuming and expensive.