Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit
Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit
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DOI：
10.1016/j.molcel.2007.05.007
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发表时间：
2007-06-08
期刊：
影响因子：
16
通讯作者：
Bjorklund, Stefan
中科院分区：
文献类型：
--
作者：
Backstrom, Stefan;Elfving, Nils;Bjorklund, Stefan
Mediator是一种转录辅助调节因子，在从酵母到人类的真核生物中都有发现，但在植物界中尚未发现。在这里，我们确定调解人在植物，拟南芥。植物介体亚基与其他物种的同源性通常很低，但我们的生化纯化鉴定出21个保守亚基和6个A。拟南芥特异性介体亚基。最值得注意的是，我们确定了A。分别作为Med14和Med25亚基的植物色素和荧光时间1（PFT 1）。这些发现表明，特定的植物介体亚基与调节专门的过程有关，例如控制细胞增殖和调节开花时间以响应光质量。植物介体的鉴定将为植物转录调控提供新的工具和见解。
Mediator, a central coregulator of transcription, has been identified as a large protein complex in eukaryotes ranging from yeast to man. It is therefore remarkable that Mediator has not yet been identified within the plant kingdom. Here we identify Mediator in a plant, Arabidopsis thaliana. The plant Mediator subunits typically show very low homology to other species, but our biochemical purification identifies 21 conserved and six A. thaliana-specific Mediator subunits. Most notably, we identify the A. thaliana proteins STRUWWELPETER (SWP) and PHYTOCHROME AND FLOWERING TIME 1 (PFT1) as the Med14 and Med25 subunits, respectively. These findings show that specific plant Mediator subunits are linked to the regulation of specialized processes such as the control of cell proliferation and the regulation of flowering time in response to light quality. The identification of the plant Mediator will provide new tools and insights into the regulation of transcription in plants.