High-Level Production of Exopolysaccharides by a Cosmic Radiation-Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes).
High-Level Production of Exopolysaccharides by a Cosmic Radiation-Induced Mutant M270 of the Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes).
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DOI：
10.1615/intjmedmushrooms.v18.i7.70
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发表时间：
2016
影响因子：
1.2
通讯作者：
Chen Zhao;Xue-Mei Tian;Guangyuan Wang;Ai-Rong Song;W. Liang
中科院分区：
文献类型：
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作者：
Chen Zhao;Xue-Mei Tian;Guangyuan Wang;Ai-Rong Song;W. Liang
利用宇宙辐射诱变技术，成功分离到一个新的灰树花（Grifola frondosa）突变体M270，该突变体具有高产量的胞外多糖（eps）。刚果红染色后发现，突变体M270与亲本菌株265相比，菌丝黏附的EPS层（~10µm）更清晰、更厚。对M270进行20 L批量发酵，发酵204 h后EPS为10.3 g/L，干菌丝生物量为17.9 g/L。此外，从M270中纯化出EPS的主要水溶性组分（EP1），确定其为杂聚糖-蛋白复合物，总碳水化合物含量为91% (w/w)，总蛋白质含量为9% （w/w）。EP1中检测到4种单糖- d -甘露糖、d -氨基葡萄糖、d -葡萄糖和d -木糖，摩尔比为17.6:1.8:100:2.5。EP1的主要成分分子量为8.9 kDa。来自M270的EPS显著抑制了小鼠肉瘤180实体瘤的生长。该灰树花M270突变体可以作为生产多糖的较好候选菌株。
A new Grifola frondosa mutant, M270, was successfully isolated for high production of exopolysaccharides (EPSs) using cosmic radiation-induced mutagenesis. We found that the mutant M270 had a clearer and thicker EPS layer (~10 µm) adhering to mycelia than those of its parent strain 265 after Congo red staining. In the 20-L batch fermentation for M270, 10.3 g/L of EPS and 17.9 g/L of dry mycelia biomass were obtained after 204 hours of fermentation. Furthermore, a main water-soluble fraction (EP1) in the EPS was purified from M270 and then confirmed to be heteroglycan-protein complex with 91% (w/w) total carbohydrates and 9% (w/w) total proteins. Four kinds of monosaccharide-D-mannose, D-glucosamine, D-glucose, and D-xylose-were detected in EP1 with a molar ratio of 17.6:1.8:100:2.5. The molecular mass of the main component in EP1 was 8.9 kDa. The EPS from M270 significantly inhibited the growth of sarcoma 180 solid tumors in mice. This G. frondosa M270 mutant could serve as a better candidate strain for polysaccharide production.