Immunocytochemical localization of relaxin in the golden hamster (Mesocricetus auratus) during the last half of gestation.
Immunocytochemical localization of relaxin in the golden hamster (Mesocricetus auratus) during the last half of gestation.
复制标题
妊娠后半期金仓鼠（Mesocricetus auratus）松弛素的免疫细胞化学定位。
DOI：
10.1095/biolreprod37.4.925
复制
发表时间：
1987
影响因子：
3.6
通讯作者：
Leavitt,WW
中科院分区：
文献类型：
--
作者：
Renegar,RH;Cobb,AD;Leavitt,WW
本研究的目的是通过免疫细胞化学技术确定松弛素在怀孕仓鼠的组织来源。在妊娠第8、10、12、14和15天从仓鼠中回收卵巢、子宫和胎盘组织，并进行光学显微镜检查。松弛素的免疫反应性定位在组织切片的抗生物素蛋白-生物素-过氧化物酶技术，使用抗血清猪松弛素。在妊娠第8天，松弛素免疫反应定位于原代巨大滋养层细胞（GTC-1 s）毗邻子宫蜕膜。在第10天，松弛素免疫反应定位于GTC-1 s，次级巨大滋养层细胞（GTC-2s）相邻的外胎盘锥，和子宫内膜粒细胞在鞘动脉壁。在第12天，松弛素免疫反应主要观察到GTC-2的胎盘滋养细胞之间的细胞，但不是在胎盘迷路的细胞散布。松弛素免疫反应性GTC的染色强度和数量在第12天和第14天之间增加，但在第15天下午减少。松弛素不局限于子宫腺或黄体。这些观察结果表明，胎盘是松弛素在怀孕仓鼠的组织来源。
The objective of this study was to determine the tissue source of relaxin in pregnant hamsters by immunocytochemical techniques. Ovarian, uterine, and placental tissues were recovered from hamsters on Days 8, 10, 12, 14, and 15 of gestation and processed for light microscopy. Relaxin immunoreactivity was localized in tissue sections by the avidin-biotin-peroxidase technique using antiserum to porcine relaxin. On Day 8 of gestation, relaxin immunoreactivity was localized in primary giant trophoblast cells (GTC-1s) adjacent to the uterine decidua. On Day 10, relaxin immunoreactivity was localized in GTC-1s, secondary giant trophoblast cells (GTC-2s) adjacent to the ectoplacental cone, and endometrial granulocytes in the wall of sheathed arteries. On Day 12, relaxin immunoreactivity was observed primarily in GTC-2s interspersed among cells of the placental trophospongium but not in cells of the placental labyrinth. The intensity of staining and number of relaxin immunoreactive GTCs increased between Days 12 and 14 but was decreased by Day 15 PM. Relaxin was not localized in uterine glands or corpora lutea. These observations suggest that the placenta is the tissue source of relaxin in pregnant hamsters.