Methyl farnesoate regulatory mechanisms underlying photoperiod-dependent sex determination in the freshwater crustacean Daphnia magna.
Methyl farnesoate regulatory mechanisms underlying photoperiod-dependent sex determination in the freshwater crustacean Daphnia magna.
复制标题
法尼索酸甲酯调节机制是淡水甲壳动物大型水蚤光周期依赖性性别决定的基础。
DOI：
10.1002/jat.4035
复制
发表时间：
2021
影响因子：
3.3
通讯作者：
Tsuyoshi Ohira.
中科院分区：
文献类型：
--
作者：
Kenji Toyota;Tomomi Sato;Taisen Iguchi;Tsuyoshi Ohira.
大型蚤（Daphnia magna）是一种重要的淡水动物，在生态毒理学研究中有着广泛的应用。在过去的20年中，已经证明，局部应用保幼激素（JH）或JH类似物的母亲水蚤诱导雄性后代的生产。基于这一发现，开发了具有JH激动作用的化学物质的体内筛选验证方法。虽然这个筛选系统成功地鉴定了一些JH样化学物质，但男性性别决定过程的分子机制仍然很大程度上未知。为了解决这个问题，我们建立了一个可靠的雄性或雌性生产系统，使用Daphnia pulex WTN 6菌株通过改变饲养光周期。利用这一饲养系统，我们成功地发现了几个涉及雄性性别决定的因素，如离子型谷氨酸受体，蛋白激酶C和泛酸。在这里，我们使用两个D。此外，还发现了一些通过光周期差异控制雌性或雄性后代产生的巨菌株，可作为生态毒理学研究的模式物种。我们证明，离子型谷氨酸受体拮抗剂或蛋白激酶C抑制剂的治疗强烈抑制雄性后代的生产，即使在雄性生产条件下。此外，我们发现，男性性别决定过程可能在D. magnaandD.根据目前的实验。本研究为JH激动剂和拮抗剂的体内筛选提供了一种良好的实验方法。此外，使用具有光周期依赖性性别决定方式的水蚤将极大地有助于了解JH在水蚤中的作用方式。
Freshwater zooplanktonDaphnia magnahas been widely used in ecotoxicology studies. During the last 20 years, it has been demonstrated that the topical application of juvenile hormone (JH) or JH analogs to mother daphnids induce male offspring production. Based on this finding, an in vivo screening validation method for chemicals with JH agonistic effect has developed. Although this screening system successfully identified a number of JH‐like chemicals, molecular mechanisms underlying the male sex‐determining process remain largely unknown. To address this issue, we established a reliable male‐ or female‐producing system usingDaphnia pulexWTN6 strain by changing the rearing photoperiod. Taking advantage of this rearing system, we successfully found several factors involving male sex determination such as ionotropic glutamate receptors, protein kinase C and pantothenate. Here, we used twoD. magnastrains that can also control the production of female or male offspring by photoperiod differences as model species for ecotoxicology studies. We demonstrated that either treatment of antagonist of ionotropic glutamate receptors or inhibitor of protein kinase C strongly suppressed male offspring production even under male‐producing conditions. Moreover, we revealed that male sex‐determining processes are likely diverged betweenD. magnaandD. pulexbased on the current experiment. This study provides a fine experimental method for in vivo screening not only JH agonists but also JH antagonists. Moreover, using daphnids with photoperiod‐dependent sex determination manner will hugely contribute to understanding the mode‐of‐action of JH in daphnids.