Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells
Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells
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DOI：
10.1016/j.bbamcr.2005.11.014
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Oguma, K
中科院分区：
文献类型：
--
作者：
Uotsu, N;Nishikawa, A;Oguma, K
细菌C型肉毒梭菌产生祖毒素（C16 S毒素），其在内化之前结合HT-29细胞表面上以唾液酸终止的O-连接的糖链[A. Nishikawa，N.鱼津湾J.C.有光李，Y。米乌拉湾，巴西-地Fujinaga，H. Nakada，T.渡边T. Ohyama，Y.坂野湾Oguma，生物化学，生物物理。通信资源319（2004）327-333] [21]。基于此，假设C16 S毒素通过网格蛋白包被的小凹内化。为了研究这种可能性，使用共聚焦激光扫描显微镜用荧光抗体观察内化的毒素。共聚焦图像清楚地表明，C16 S毒素主要通过网格蛋白包被的小凹内化，并定位于早期内体。然而，毒素与小窝的组分之一小窝蛋白-1共定位，这意味着毒素也通过小窝内化。共聚焦图像还显示，神经毒素转运到内体被转移到高尔基体。然而，无毒组分没有与高尔基体标记蛋白TGN 38融合，这意味着神经毒素在内体中与祖毒素分离。这些结果表明，C16 S毒素在核内体中被分离成神经毒素和其他蛋白质，神经毒素进一步被转移到高尔基体，高尔基体是蛋白质分选的中心。(c)2005 Elsevier B. V.保留所有权利。
The bacterium Clostridium botulinum type C produces a progenitor toxin (C16S toxin) that binds to O-linked sugar chains terminating with sialic acid on the surface of HT-29 cells prior to internalization [A. Nishikawa, N. Uotsu, H. Arimitsu, J.C. Lee, Y. Miura, Y. Fujinaga, H. Nakada, T. Watanabe, T. Ohyama, Y. Sakano, K. Oguma, Biochem. Biophys. Res. Commun. 319 (2004) 327-333] [21] . Based on this, it was hypothesized that the C16S toxin is internalized via clathrin-coated pits. To examine this possibility, the internalized toxin was observed with a fluorescent antibody using confocal laser-scanning microscopy. The confocal images clearly indicated that the C16S toxin was internalized mainly via clathrin-coated pits and localized in early endosomes. The toxin was colocalized with caveolin-1 which is one of the components of caveolae, however, implying the toxin was also internalized via caveolae. The confocal images also showed that the neurotoxin transported to the endosome was transferred to the Golgi apparatus. However, the non-toxic components were not merged with the Golgi marker protein, TGN38, implying the neurotoxin was dissociated from progenitor toxin in endosomes. These results suggested that the C16S toxin was separated to the neurotoxin and other proteins in endosome and the neurotoxin was further transferred to the Golgi apparatus which is the center for protein sorting. (c) 2005 Elsevier B.V. All rights reserved.