In vivo protein transduction: Delivery of a biologically active protein into the mouse
In vivo protein transduction: Delivery of a biologically active protein into the mouse
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DOI：
10.1126/science.285.5433.1569
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发表时间：
1999-09-03
期刊：
影响因子：
56.9
通讯作者：
Dowdy, SF
中科院分区：
文献类型：
--
作者：
Schwarze, SR;Ho, A;Dowdy, SF
将治疗性蛋白质递送到组织中并穿过血脑屏障受到蛋白质的大小和生化性质的严重限制。这里显示，腹膜内注射与来自人免疫缺陷病毒达特蛋白的蛋白转导结构域融合的120千道尔顿β-半乳糖苷酶蛋白，导致生物活性融合蛋白递送至小鼠的所有组织，包括脑。这些结果为在蛋白质治疗的背景下将蛋白质直接递送到患者体内以及用模式生物进行表观遗传实验开辟了新的可能性。
Delivery of therapeutic proteins into tissues and across the blood-brain barrier is severely limited by the size and biochemical properties of the proteins. Here it is shown that intraperitoneal injection of the 120-kilodalton beta-galactosidase protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in-delivery of the biologically active fusion protein to all tissues in mice, including the brain. These results open new possibilities for direct delivery of proteins into patients in the context of protein therapy, as well as for epigenetic experimentation with model organisms.