Keratin and involucrin expression in discoid lupus erythematosus and lichen planus
Keratin and involucrin expression in discoid lupus erythematosus and lichen planus
复制标题
DOI：
10.1007/s004030050233
复制
发表时间：
1997-08-01
影响因子：
3
通讯作者：
Takahashi, H
中科院分区：
文献类型：
--
作者：
Ichikawa, E;Watanabe, S;Takahashi, H
为了更好地了解盘状红斑狼疮和扁平苔藓皮损中表皮细胞的异常分化或成熟，本研究对盘状红斑狼疮和扁平苔藓皮损中角蛋白和外皮蛋白的表达进行了研究。使用一组单克隆抗角蛋白抗体和多克隆抗外皮蛋白抗体，并使用三种抗角蛋白抗体通过一维和二维凝胶电泳和免疫印迹分析各分析五个样本，分化特异性角蛋白的表达与正常表皮相似，外皮蛋白甚至在棘层下部表达，角蛋白6和16是过度增殖状态的特征性标志物，角蛋白6、16和17在非过度增生和萎缩的表皮中表达，真皮中有水肿变性和炎性浸润。这些结果表明，角蛋白6、16和17在盘状红斑狼疮和扁平苔藓中的表达可能反映了对基底细胞层损伤的创伤愈合反应，或者可以在由真皮中的浸润炎性细胞产生的细胞因子的控制下。
In the present study, keratin and involucrin expression were studied in cutaneous lesions of discoid lupus erythematosus and lichen planus in order to gain a better understanding of the abnormal differentiation or maturation of the epidermal cells in these dermatoses, Ten specimens each from discoid lupus erythematosus and lichen planus were analyzed by immunohistochemical techniques, using a panel of monoclonal antikeratin antibodies and polyclonal anti-involucrin antibody, and five specimens each were analyzed by one-and two-dimensional gel electrophoresis and immunoblot analysis using three antikeratin antibodies, No significant difference was found between the dermatoses, The expression of differentiation-specific keratins showed a similar pattern to that in normal epidermis, and involucrin was expressed even in the lower part of the stratum spinosum, Keratins 6 and 16, which are characteristic markers of hyperproliferative states, and keratin 17 were detected in nonhyperproliferative and atrophic epidermis with hydropic degeneration and inflammatory infiltrates in the dermis, These results suggest that expression of keratins 6, 16 and 17 in discoid lupus erythematosus and lichen planus may reflect a wound healing response to the damage to the basal cell layer, or may be under the control of cytokines produced by infiltrating inflammatory cells in the dermis.