Participation of water in Hin recombinase--DNA recognition.
Participation of water in Hin recombinase--DNA recognition.
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水参与 Hin 重组酶——DNA 识别。
DOI：
10.1002/pro.5560051019
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Sligar,SG
中科院分区：
文献类型：
--
作者：
Robinson,CR;Sligar,SG
通过分析在不同浓度的中性溶质和助溶剂存在下的解离常数，发现水分子参与了HIN重组酶与其操纵子DNA的相互作用。凝胶迁移率移动法测定的解离常数随着二甲基亚砜、甘油、蔗糖或聚乙二醇浓度的增加而增大。渗透压是唯一与所有化合物的离解常数变化相关的性质。这一数据表明，一小部分水分子的结合伴随着Hin-DNA复合体的形成，并指出了溶剂分子在协助DNA结合蛋白与其同源DNA序列之间的位点特异性相互作用中的新作用。
The participation of water molecules in the interaction between the Hin recombinase and its operator DNA has been detected by analysis of the dissociation constant in the presence of varying concentrations of neutral solutes and cosolvents. The dissociation constant as measured by gel mobility shift assays increased as the concentration of dimethyl sulfoxide, glycerol, sucrose, or polyethylene glycol was increased. Osmotic pressure is the only property that correlates with the change in the dissociation constant for all compounds. This data indicates that binding of a small population of water molecules accompanies formation of the Hin‐DNA complex, and points to a novel role for solvent molecules in assisting site specific interaction between DNA‐binding proteins and their cognate DNA sequence.