Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii.
Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii.
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DOI：
10.1083/jcb.98.1.229
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发表时间：
1984-01
期刊：
影响因子：
--
通讯作者：
Luck DJ
中科院分区：
文献类型：
--
作者：
Dutcher SK;Huang B;Luck DJ
导致鞭毛移动性改变的两个基因座的突变影响了莱茵衣藻鞭毛的中心微管复合体。这两个位点的突变主要影响复合体的C1微管。在分离的轴丝中，PF16基因座上的三个等位基因影响C1微管的稳定性。这种表型使我们能够确定至少有10个中央对复合体的多肽是C1微管所特有的。运动性缺陷与未能在体内组装这十种多肽中的三种有关。通过对5个基因内回复突变体的分析和对双核细胞拯救实验中轴丝的分析，表明PF16基因座的结构基因产物是一种多肽，分子量为57000。Pf6基因座上的三个等位基因影响C1微管两个突起中的一个的组装，这个突起至少由三个多肽组成，它们是分离的pf16轴丝中缺失的多肽的子集。尚未鉴定出PF6基因座的结构基因产物。该基因产物可能不是双核细胞拯救实验中所鉴定的投射成分之一。对分离的野生型轴丝的化学提取表明，至少有7种多肽成分是C2微管所特有的。
Mutations at two loci, which cause an altered mobility of the flagella, affected the central pair microtubule complex of Chlamydomonas reinhardtii flagella. The mutations at both loci primarily affected the C1 microtubule of the complex. Three alleles at the PF16 locus affected the stability of the C1 microtubule in isolated axonemes. This phenotype has allowed us to determine that at least ten polypeptides of the central pair complex are unique to the C1 microtubule. The motility defect was correlated with the failure to assemble three of these ten polypeptides in vivo. The structural gene product of the PF16 locus was a polypeptide with molecular weight 57,000 as shown by analysis of five intragenic revertants and by analysis of axonemes from dikaryon rescue experiments. Three alleles at the PF6 locus affected the assembly of one of the two projections of the C1 microtubule and this projection was formed by at least three polypeptide components, which are a subset of polypeptides missing in isolated pf16 axonemes. No structural gene product has been identified for the PF6 locus. The gene product is probably not one of the identified projection constituents as shown by analysis of dikaryon rescue experiments. Chemical extraction of isolated wild-type axonemes suggests that at least seven polypeptide components are unique to the C2 microtubule.