Enrichment and characterization of acid-tolerant nitrifying sludge
Enrichment and characterization of acid-tolerant nitrifying sludge
复制标题
耐酸硝化污泥的富集和表征
DOI：
10.1016/j.jenvman.2016.09.069
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发表时间：
2016
影响因子：
8.7
通讯作者：
Yunhong Zhang
中科院分区：
文献类型：
--
作者：
Lan Wang;Ping Zheng;Ghulam Abbas;Yajuan Xing;Wei Li;Ru Wang;Liangwei Deng;Yunhui Lei;Dan Zheng;Yunhong Zhang
硝化作用是一种酸化过程，由于大多数氨氧化细菌的嗜碱性质，因此需要添加外部碱度。在这项研究中，好氧活性污泥被用作接种物的内循环气升式反应器中，这导致了成功的富集耐酸硝化（ACIN）污泥在pH 6.0的果园土壤悬浮液顺序加入。结果表明，ACIN污泥具有显著的耐酸能力，其体积氨转化率为1.13 kg N m-3day-1。在pH 6.0时，ACIN污泥的最大比氨转化率（0.29 g N g−1VSS day−1）高于嗜酸性硝化污泥（0.14 g N g−1VSS day−1），并且对pH波动具有良好的抵抗力，在pH 7.5时具有最大比氨转化率（0.584 g N g−1VSS day−1）。微生物群落分析表明，较高丰度的耐酸亚硝化螺旋菌和氨氧化古菌为ACIN污泥显著的耐酸能力奠定了坚实的基础。
Nitrification is an acidifying process that requires the addition of external alkalinity because of the alkaliphilic nature of the most ammonia-oxidizing bacteria. In this study, aerobic activated sludge was used as inoculum in an internal loop air-lift reactor, which resulted in successful enrichment of acid-tolerant nitrifying (ACIN) sludge at pH 6.0 by sequential addition of tea orchard soil suspension. The results showed that ACIN sludge had a remarkable acid tolerant capability with a volumetric ammonia conversion rate of 1.13 kg N m−3day−1. ACIN sludge showed a higher maximum specific ammonia conversion rate (0.29 g N g−1VSS day−1) than neutrophilic nitrifying sludge (0.14 g N g−1VSS day−1) at pH 6.0 and had good resistance against pH fluctuations, with a maximum specific ammonia conversion rate (0.584 g N g−1VSS day−1) at pH 7.5. Microbial community analysis indicated that the higher abundance of acid tolerantNitrosospiraand ammonia-oxidizing archaea laid a solid foundation for the remarkable acid-tolerant capability of ACIN sludge.