Peroxiredoxin 4 improved aging-related delayed wound healing in mice.
Peroxiredoxin 4 improved aging-related delayed wound healing in mice.
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DOI：
10.1016/j.jid.2021.04.015
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发表时间：
2021-05
期刊：
影响因子：
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通讯作者：
R. Yamaguchi;Xin Guo;Jianbo Zheng;Jing Zhang;Jia Han;A. Shioya;H. Uramoto;T. Mochizuki;S. Yamada
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文献类型：
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作者：
R. Yamaguchi;Xin Guo;Jianbo Zheng;Jing Zhang;Jia Han;A. Shioya;H. Uramoto;T. Mochizuki;S. Yamada
与衰老相关的伤口愈合延迟是全球关注的问题。氧化应激参与伤口愈合。抗氧化酶在这一过程中发挥着各种作用。PRDX 4是PRDX家族的成员，在损伤后上调。为了研究PRDX 4对衰老相关的伤口愈合的影响，我们将C57 BL/6 J（野生型）、人Prdx 4 β转基因（即，hPrdx 4 +/+），Prdx 4-敲除（即，Prdx 4-/y）小鼠的皮肤创伤形成。PRDX 4的过表达加速了成年和老年小鼠的伤口愈合，但在年轻小鼠中没有。DHPrdx 4 +/+小鼠表现出氧化应激和炎症减少、嗜中性粒细胞数量减少、巨噬细胞浸润增加、血管生成增加和GF水平增加。成年和老年hPrdx 4 +/+小鼠的肉芽组织中的成纤维细胞比匹配的野生型小鼠中的成纤维细胞更丰富。PRDX 4缺乏与成年和老年小鼠的死亡率相关。在体外，PRDX 4的过表达促进了成年或老年小鼠来源的成纤维细胞的增殖和迁移，并使成纤维细胞对过氧化氢的细胞毒性更具抵抗力。PRDX 4对伤口愈合至关重要，可以从多个方面改善愈合过程，这表明它可能对伤口治疗非常有益，特别是对老年人。
Aging-related delayed wound healing is an issue of concern worldwide. Oxidative stress is involved in wound healing. Antioxidative enzymes have various roles in this process. PRDX4, a member of the PRDX family, is upregulated after injury. To investigate the effects of PRDX4 on aging-related wound healing, we subjected C57BL/6J (wild-type), humanPrdx4‒transgenic (i.e.,hPrdx4+/+),Prdx4-knockout (i.e.,Prdx4–/y) mice of three age groups (young, adult, and aged) to skin wound formation. The overexpression of PRDX4 accelerated wound healing in adult and aged mice but not in young mice. AgedhPrdx4+/+mice showed reduced oxidative stress and inflammation, lower numbers of neutrophils, increased macrophage infiltration, increased angiogenesis, and increased GF levels. The granulation tissue of adult and agedhPrdx4+/+mice was richer in fibroblasts than that in the matched wild-type mice. PRDX4 deficiency was associated with mortality in adult and aged mice. In vitro, the overexpression of PRDX4 promoted the proliferation and migration of fibroblasts derived from adult or aged mice and made fibroblasts more resistant to the cytotoxicity of hydrogen peroxide. PRDX4 is essential for wound healing and can improve the healing process from multiple aspects, suggesting that it may be very beneficial to wound treatment, especially for the elderly.