Plastic film carbon electrodes: enzymatic modification for on-line, continuous, and simultaneous measurement of lactate and glucose using microdialysis sampling.
Plastic film carbon electrodes: enzymatic modification for on-line, continuous, and simultaneous measurement of lactate and glucose using microdialysis sampling.
复制标题
塑料膜碳电极：酶促修饰，使用微透析取样在线、连续、同时测量乳酸和葡萄糖。
DOI：
10.1021/ac9706990
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发表时间：
1998
影响因子：
7.4
通讯作者：
Katsunobu Yamamoto
中科院分区：
文献类型：
--
作者：
Osborne Pg;O. Niwa;Katsunobu Yamamoto
制备了环形和裂片塑料薄膜碳电极（PFCEs），用于耦合到微透析取样系统的薄层径向流电池。pfce最初被锇聚（乙烯吡啶）氧化还原聚合物辣根过氧化物酶（Os-gel-HRP）包裹。然后用第二层氧化酶产生酶双分子层（氧化酶/ os -凝胶- hrp） pfce，随后用醋酸纤维素覆盖pfce，用于在0 mV （vs Ag/AgCl）下测定葡萄糖或乳酸。分裂盘电极的几何结构使得不同的氧化酶固定在分裂盘的每一半上，os -凝胶-酶标涂覆，PFCE，以方便从单个透析液中连续在线测定这两种分析物的电化学独立。在连续流动实验中，醋酸纤维素包被氧化酶/ os -凝胶- hrp铸包的pfce能够快速稳定背景电流，并对底物表现出线性和敏感的响应。抗坏血酸的影响是最小的，并且在伴侣分裂盘电极之间的串扰被证明是可以接受的体内应用。通过对清醒大鼠透析液纹状体细胞外葡萄糖和乳酸变化的在线连续定量分析(a)通过灌注去极化剂Veratridine局部刺激神经元和(b)物理约束，证明了该分析系统的效用。
Ring and split-disk plastic film carbon electrodes (PFCEs) were fabricated for use in thin-layer radial flow cells which were coupled to a microdialysis sampling system. PFCEs, were initially coated with osmium poly(vinylpyridine) redox polymer horseradish peroxidase (Os-gel-HRP). Then a second coat of oxidase enzyme was applied to produce enzyme bilayer (oxidase/Os-gel-HRP) PFCEs which were subsequently over-coated with cellulose acetate for use in the determination of glucose or lactate at 0 mV (vs Ag/AgCl). Split-disk electrode geometry enabled different oxidase enzymes to be immobilized on each half of a split-disk, Os-gel-HRP-coated, PFCE to facilitate the electrochemically independent yet continuous on-line determination of these two analytes from a single dialysate. In continuous-flow experiments, cellulose acetate overcoated oxidase/Os-gel-HRP cast-coated PFCEs were quick to stabilize background current and displayed linear and sensitive responses to substrates. The effect of ascorbic acid was minimal and cross talk between partner split-disk electrodes was demonstrated to be acceptable for in vivo applications. The utility of this analytical system is demonstrated by the quantitative on-line continuous assay of changes in dialysate striatal extracellular glucose and lactate from a conscious rat during (a) local stimulation of neurons by perfusion with the depolarizing agent, Veratridine, and (b) physical restraint.