Effect of Storage Method and Associated Holding Time on Nitrogen and Phosphorus Concentrations in Surface Water Samples
Effect of Storage Method and Associated Holding Time on Nitrogen and Phosphorus Concentrations in Surface Water Samples
复制标题
储存方法和相关保存时间对地表水样品中氮和磷浓度的影响
DOI：
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发表时间：
2013
影响因子：
2.7
通讯作者：
M. Locke
中科院分区：
文献类型：
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作者：
M. Moore;M. Locke
进行了评估，以确定样品储存方法和相关的保存时间对现场地表水营养物质浓度的影响。在一年的时间里，对六个地表水点和两个营养物加标的实验室水样进行了硝酸盐、亚硝酸盐、铵、过滤后的正磷和总正磷浓度的评估。在进行营养分析之前，将样品在室温（23°C）下保存24小时，结果中有18%±2%的结果与对照组（收集后立即进行分析）显著不同。在营养分析之前，将样品放在冷却器（4°C）中7天，结果导致30%±1%的值与对照组显着不同。在分析前，样品在- 20°C的冷冻室中放置7天，分别产生34%±12%、44%±10%和28%±5.7%的铵、过滤后的正磷酸盐和总正磷酸盐，这些值与对照组显著不同。这项研究突出了研究人员在有效收集、储存和营养分析样品方面面临的挑战，特别是当地点遥远且难以到达时。
Assessments were conducted to determine the effect of sample storage method and associated holding time on surface water nutrient concentrations from field sites. Six surface water sites and two nutrient spiked, laboratory water samples were evaluated for nitrate, nitrite, ammonium, filtered orthophosphorus, and total orthophosphorus concentrations on four separate days throughout the period of 1 year. Samples stored at ambient temperature (23°C) for 24 h prior to nutrient analyses resulted in 18 % ± 2 % of results being significantly different from controls (which were analyzed immediately upon collection). Samples placed in the cooler (4°C) for 7 days prior to nutrient analyses resulted in 30 % ± 1 % of values being significantly different from controls. Samples placed in the freezer (−20°C) for 7 days prior to analyses resulted in 34 % ± 12 %, 44 % ± 10 %, and 28 % ± 5.7 % of ammonium, filtered orthophosphate, and total orthophosphate, respectively, values being significantly different from controls. This study highlights the challenges facing researchers in efficient collection, storage and nutrient analysis of samples, especially when sites are remote and difficult to access .