Microbial hydrogen production from sewage sludge bioaugmented with a constructed microbial consortium
Microbial hydrogen production from sewage sludge bioaugmented with a constructed microbial consortium
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DOI：
10.1016/j.ijhydene.2010.03.059
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发表时间：
2010-10-01
影响因子：
7.2
通讯作者：
Das, Debabrata
中科院分区：
文献类型：
--
作者：
Kotay, Shireen Meher;Das, Debabrata
由3株兼性产H-2厌氧细菌（阴沟肠杆菌IIT-BT 08、弗劳地柠檬酸杆菌IIT-BT L139和凝结芽孢杆菌IIT-BT Si）组成的复合菌群，经发酵培养，获得了产H-2厌氧细菌。将该聚生体作为H-2生产的种子培养物进行测试。在最初的研究中，确定的培养基（MYG），E。Clobacillus的H-2产量高于其他两株菌，而且在使用聚生体时，Clobacillus也是优势菌。另一方面，B。以污泥为底物时，凝结多糖的H-2产量（37.16mlH-2/gCOD）高于其它两株菌。污泥的预处理包括灭菌（15%v/v），稀释和补充0.5%w/v葡萄糖，这被认为是必要的筛选出H-2消耗菌和改善H-2的生产。当以1：1：1的比例接种时，H-2的去除率最高，为41.23ml H-2/g COD。对废污泥的微生物谱分析表明，B.在构建的产氢菌群中，凝结多糖是主要的产氢菌。H-2产量的增加表明，在财团中，底物利用率显着较高。预处理污泥的H-2产率（35.54 ml H-2/g污泥）相对高于文献报道的（8.1-16.9 ml H-2/g污泥）。利用组合微生物菌群进行生物制氢是提高污泥产氢率的一种成功尝试。(C)2010年教授T。Nejat Veziroglu。由Elsevier Ltd.出版。保留所有权利。
A constructed microbial consortium was formulated from three facultative H-2-producing anaerobic bacteria, Enterobacter cloacae IIT-BT 08, Citrobacter freundii IIT-BT L139 and Bacillus coagulans IIT-BT Si. This consortium was tested as the seed culture for H-2 production. In the initial studies with defined medium (MYG), E. cloacae produced more H-2 than the other two strains and it also was found to be the dominant member when consortium was used. On the other hand, B. coagulans as a pure culture gave better H-2 yield (37.16 ml H-2/g CODconsumed) than the other two strains using sewage sludge as substrate. The pretreatment of sludge included sterilization (15% v/v), dilution and supplementation with 0.5% w/v glucose, which was found to be essential to screen out the H-2 consuming bacteria and ameliorate the H-2 production. Considering (1:1:1) defined consortium as inoculum, COD reduction was higher and yield of H-2 was recorded to be 41.23 ml H-2/g CODreduced. Microbial profiling of the spent sludge showed that B. coagulans was the dominant member in the constructed consortium contributing towards H-2 production. Increase in H-2 yield indicated that in consortium, the substrate utilization was significantly higher. The H-2 yield from pretreated sludge (35.54 ml H-2/g sludge) was comparatively higher than that reported in literature (8.1-16.9 ml H-2/g sludge). Employing formulated microbial consortium for biohydrogen production is a successful attempt to augment the H-2 yield from sewage sludge. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.