Taxonomic Diversity of Pico-/Nanoeukaryotes Is Related to Dissolved Oxygen and Productivity, but Functional Composition Is Shaped by Limiting Nutrients in Eutrophic Coastal Oceans.
Taxonomic Diversity of Pico-/Nanoeukaryotes Is Related to Dissolved Oxygen and Productivity, but Functional Composition Is Shaped by Limiting Nutrients in Eutrophic Coastal Oceans.
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Pico-/nanoeukaryotes的分类多样性与溶解的氧和生产力有关，但是功能组成是通过限制富营养化沿海海洋中的养分来影响的。
DOI：
10.3389/fmicb.2020.601037
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发表时间：
2020
影响因子：
5.2
通讯作者：
Gong J
中科院分区：
文献类型：
--
作者：
Wang Y;Li G;Shi F;Dong J;Gentekaki E;Zou S;Zhu P;Zhang X;Gong J
皮科-/纳米真核生物（P/内斯）是海洋微生物循环中重要的生物地球化学和生态学角色。除了大小的差异，这些小尺寸的部分可以从微型浮游生物的某些功能和生态特征。然而，关于其分类和功能多样性以及沿海海洋生态系统中沿着环境梯度的群落组成的模式的资料很少。本研究采用18 S rRNA基因高通量测序技术，对2011年夏季和冬季中国北方600 km范围内的渤海和北黄海表层沃茨中P/内斯的物种丰富度和群落组成进行了分析。操作分类单位（OTUs）的丰富度与叶绿素a（Chl-a，初级生产力的代理）浓度呈U型关系，但与溶解氧（DO）浓度呈较强的负相关关系。这两个因素也显着共同变化与基于OTU的社区组成的P/内斯。地理距离对群落组成的影响可以忽略不计。在由营养性状定义的3个功能群中，异养菌的OTU丰富度最高，与DO和Chl-a均呈U型关系。从读数来看，P/内斯群落以异养菌和兼养菌为主，这表现出沿着磷酸盐梯度的权衡，但沿着DO和Chl-a梯度没有显着变化，表明功能冗余。同样，光养生物的比例与硅酸盐的浓度呈显著正相关。我们的研究结果表明，分类和功能组成的P/内斯解耦的区域尺度上，限制营养物质是重要的因素，在调制功能组成的这些微生物在研究区域。这些发现有助于更好地了解小型真核生物的多样性及其功能在沿海海洋中的结构以及环境变化对结构化过程的影响。
Pico-/nanoeukaryotes (P/NEs) comprise both primary producers and bacterial predators, playing important biogeochemical and ecological roles in the marine microbial loop. Besides the difference in size, these small-sized fractions can be distinguished from microplankton by certain functional and ecological traits. Nevertheless, little information is available regarding patterns of their taxonomic and functional diversity and community composition along environmental gradients in coastal marine ecosystems. In this study, we applied high-throughput sequencing of 18S rRNA gene to assess the taxonomic species richness and community composition of P/NEs in surface waters of Bohai Sea and North Yellow Sea, northern China spanning a 600-km distance during summer and winter of 2011. The richness of operational taxonomic units (OTUs) formed a U-shaped relationship with concentration of chlorophyll a (Chl-a, a proxy of primary productivity), but a stronger, negative relationship with concentration of dissolved oxygen (DO). These two factors also significantly co-varied with the OTU-based community composition of P/NEs. The effect of geographic distance on community composition of P/NEs was negligible. Among the three functional groups defined by trophic traits, heterotrophs had the highest OTU richness, which exhibited a U-shaped relationship with both DO and Chl-a. The community of P/NEs was dominated by heterotrophs and mixotrophs in terms of read numbers, which showed a trade-off along the gradient of phosphate, but no significant changes along DO and Chl-a gradients, indicating functional redundancy. Similarly, the proportion of phototrophs was significantly and positively correlated with the concentration of silicate. Our results indicate that taxonomic and functional composition of P/NEs are decoupled on a regional scale, and limiting nutrients are important factors in modulating functional composition of these microorganisms in the studied area. These findings contribute toward gaining a better understanding of how diversity of small eukaryotes and their functions are structured in coastal oceans and the effect of environmental changes on the structuring process.
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影响因子：
5.1
作者：
Casamayor, EO;Massana, R;Pedrós-Alió, C
通讯作者：
Pedrós-Alió, C
影响因子：
2.5
作者：
COLE, JJ;FINDLAY, S;PACE, ML
通讯作者：
PACE, ML
影响因子：
2.1
作者：
Gong, Fahui;Li, Guihao;Dolan, John
通讯作者：
Dolan, John
影响因子：
1.7
作者：
HARRISON, WG
通讯作者：
HARRISON, WG
影响因子：
2.5
作者：
LINLEY, EAS;NEWELL, RC;LUCAS, MI
通讯作者：
LUCAS, MI