EVOLUTION OF CELL-MEDIATED ANTITUMOR IMMUNITY IN MICE BEARING A SYNGENIC CHEMICALLY INDUCED TUMOR - INFLUENCE OF TUMOR GROWTH, SURGICAL REMOVAL AND TREATMENT WITH IRRADIATED TUMOR-CELLS
EVOLUTION OF CELL-MEDIATED ANTITUMOR IMMUNITY IN MICE BEARING A SYNGENIC CHEMICALLY INDUCED TUMOR - INFLUENCE OF TUMOR GROWTH, SURGICAL REMOVAL AND TREATMENT WITH IRRADIATED TUMOR-CELLS
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DOI：
10.1002/ijc.2910090302
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发表时间：
1972-01-01
影响因子：
6.4
通讯作者：
THONIER, M
中科院分区：
文献类型：
--
作者：
BELEHRAD.J;BARSKI, G;THONIER, M
研究了C57BL/6小鼠携带化学诱导的同源肉瘤--Tbl-CL2肉瘤时，特定细胞免疫状态的演变。作为免疫活性细胞来源的腹膜细胞(PC)重复地从同一组小鼠中获得，在对靶细胞TBL CL2的体外集落生长抑制试验(CGI)中，证实了我们之前通过其他肿瘤-宿主模型的研究报道的数据，观察到了细胞介导的免疫状态的特征演变。肿瘤接种10天后，荷瘤小鼠的PC在CGI试验中对肿瘤靶细胞有较强的抑制作用。然而，这种反应很快就伴随着PC的“日食”状态。无论何时检查，这种状态一直持续到肿瘤携带者死亡。相反，接种后6周手术切除肿瘤导致PC反应性在大约2个月内重新出现。在接受术后注射TBL CL2肿瘤照射细胞的动物中，这一时间明显缩短。一项对肿瘤移植排斥反应的体内研究显示，与CGI测试有相当程度的相似之处：在携带第一个肿瘤并激活PC的动物中，肿瘤移植被排斥10天；在携带巨大肿瘤且PC处于“蚀”状态的动物中，肿瘤移植未被排斥。
The evolution of the specific cell‐mediated immune status was studied in C57BL/6 mice bearing a chemically induced syngeneic sarcoma, the TBL CL2 tumor. Peritoneal cells (PC) as the source of immunocompetent cells were repeatedly harvested from the same groups of mice checked in the in vitro colony growth inhibition tests (CGI) against target TBL CL2 cells.Confirming our data previously reported from studies with other tumor‐host models, a characteristic evolution of the cell‐mediated immune status was observed. Ten days after tumor inoculation, the PC from the tumor‐bearing mice strongly inhibited the tumor target cells in the CGI test. This reactivity was, however, soon followed by an “eclipse” state of PC. This state continued, whenever checked, until the tumor‐bearer's death. On the contrary, surgical removal of the tumor 6 weeks after inoculation led to a reappearance of PC reactivity within about 2 months. This period was clearly shortened in animals receiving a post‐operative injection of irradiated cells of the TBL CL2 tumor. An in vivo study of tumor‐graft rejection revealed a fair degree of parallelism with the CGI tests: the tumor graft was rejected in animals bearing the first tumor for 10 days and having activated PC; the tumor graft was not rejected in animals bearing huge tumors and having PC in the “eclipsed” state.