Engineering genetic circuit interactions within and between synthetic minimal cells.
Engineering genetic circuit interactions within and between synthetic minimal cells.
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DOI：
10.1038/nchem.2644
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发表时间：
2017-05
期刊：
影响因子：
21.8
通讯作者：
Boyden ES
中科院分区：
文献类型：
--
作者：
Adamala KP;Martin-Alarcon DA;Guthrie-Honea KR;Boyden ES
遗传电路和反应级联对于合成生物学、生物化学和生物工程具有重要意义。一个悬而未决的问题是如何最大限度地提高其设计的模块化，以实现不同反应网络的集成，并优化其可扩展性和灵活性。一种选择是封装在脂质体内，这使得化学反应能够在良好隔离的环境中进行。在这里，我们调整脂质体封装，使模块化，控制区室化的遗传电路和级联。我们证明，它是可能的工程基因电路含有合成的最小细胞（synells），包含多部分的遗传级联，这些级联可以控制外部信号以及脂质体间的通信没有串扰。我们还表明，含有不同级联的脂质体可以以受控的方式融合，使不相容反应的产物可以聚集在一起。因此，合成细胞能够更模块化地创建合成生物学级联，这是迈向其最终可编程性的重要一步。
Genetic circuits and reaction cascades are of great importance for synthetic biology, biochemistry, and bioengineering. An open question is how to maximize the modularity of their design to enable the integration of different reaction networks and to optimize their scalability and flexibility. One option is encapsulation within liposomes which enables chemical reactions to proceed in well-isolated environments. Here we adapt liposome encapsulation to enable the modular, controlled compartmentalization of genetic circuits and cascades. We demonstrate that it is possible to engineer genetic circuit-containing synthetic minimal cells (synells) to contain multiple-part genetic cascades, and that these cascades can be controlled by external signals as well as inter-liposomal communication without cross-talk. We also show that liposomes containing different cascades can be fused in a controlled way so that the products of incompatible reactions can be brought together. Synells thus enable more modular creation of synthetic biology cascades, an essential step towards their ultimate programmability.