Confocal Brillouin microscopy for three-dimensional mechanical imaging
Confocal Brillouin microscopy for three-dimensional mechanical imaging
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DOI：
10.1038/nphoton.2007.250
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发表时间：
2008-01-01
期刊：
影响因子：
35
通讯作者：
Yun, Seok Hyun
中科院分区：
文献类型：
--
作者：
Scarcelli, Giuliano;Yun, Seok Hyun
1922年由布里渊（Brillouin）首次报道的声致非弹性光散射（1）允许非接触地直接读出材料的粘弹性性质，并且已经被广泛研究用于材料表征2、结构监测3和环境传感。将布里渊技术从点采样光谱扩展到成像模式（5）将为机械成像开辟新的可能性，但由于需要快速光谱采集而具有挑战性。在这里，我们展示了一个共焦布里渊显微镜的基础上，一个完全平行的光谱仪，一个虚拟成像的相控阵列，提高了近100倍的检测效率比以前的方法。使用该系统，我们显示了第一个横截面布里渊成像的基础上的弹性特性作为对比机制，并监测聚合物交联过程中弹性模量的快速动态变化。此外，我们报告的第一个在原位生物力学测量的晶体透镜在小鼠眼睛。这些结果表明布里渊显微镜在生物医学和生物材料科学中的多种应用。
Acoustically induced inelastic light scattering, first reported in 1922 by Brillouin(1), allows non-contact, direct readout of the viscoelastic properties of a material and has widely been investigated for material characterization 2, structural monitoring 3 and environmental sensing'. Extending the Brillouin technique from point sampling spectroscopy to imaging modality(5) would open up new possibilities for mechanical imaging, but has been challenging because rapid spectrum acquisition is required. Here, we demonstrate a confocal Brillouin microscope based on a fully parallel spectrometer-a virtually imaged phased array-that improves the detection efficiency by nearly 100-fold over previous approaches. Using the system, we show the first cross-sectional Brillouin imaging based on elastic properties as the contrast mechanism and monitor fast dynamic changes in elastic modulus during polymer crosslinking. Furthermore, we report the first in situ biomechanical measurement of the crystalline lens in a mouse eye. These results suggest multiple applications of Brillouin microscopy in biomedical and biomaterial science.