Anti-P-selectin antibody attenuates rat brain ischemic injury
Anti-P-selectin antibody attenuates rat brain ischemic injury
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DOI：
10.1016/s0304-3940(99)00229-3
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发表时间：
1999-04-23
影响因子：
2.5
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Suzuki, H;Hayashi, T;Abe, K
观察抗p -选择素单克隆抗体对大鼠大脑中动脉闭塞24 h缺血性脑损伤的保护作用。MCAO前5min静脉注射抗大鼠p选择素单克隆抗体，剂量为1 mg/kg。对照动物接受相同体积的车辆。MCAO通过尼龙线插入硅胶涂层24小时完成。抗p -选择素抗体的应用显著降低了MCAO 24小时的梗死面积和脑含水量。虽然正常情况下未检测到白细胞浸润，但在MCAO第1天变得非常明显。然而，用ARP 2-4治疗可显著减少白细胞数量。这些结果表明，给药抗p -选择素单克隆抗体可减轻梗死面积和脑水肿，这与白细胞浸润减少有关。1999爱思唯尔科学爱尔兰有限公司版权所有。
We examined a protective effect of anti-P-selectin monoclonal antibody against rat ischemic brain injury with 24 h of middle cerebral artery occlusion (MCAO). Anti-rat P-selectin monoclonal antibody, was intravenously injected at a dose of 1 mg/kg at 5 min before MCAO. Control animals received the same volume of vehicle. MCAO was accomplished by an insertion of nylon thread with silicone coating for 24 h. Application of anti-P-selectin antibody significantly reduced infarct size and brain water content at 24 h of MCAO. Although leukocyte infiltration was not normally detected, it became remarkably evident at 1 day of MCAO. However, treatment with ARP 2-4 significantly reduced the number of leukocytes. These results demonstrated that administration of monoclonal antibody against P-selectin attenuated infarct size and brain edema, which was associated with reduction of leukocyte infiltration. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.