Anti‐neutrophil cytoplasmic antibodies (ANCA) in inflammatory bowel disease: characterization and clinical correlates
Anti‐neutrophil cytoplasmic antibodies (ANCA) in inflammatory bowel disease: characterization and clinical correlates
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炎症性肠病中的抗中性粒细胞胞浆抗体（ANCA）：特征和临床相关性
DOI：
10.1111/j.1365-2249.1994.tb07024.x
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发表时间：
1994
影响因子：
4.6
通讯作者：
Cornelis Kallenberg
中科院分区：
文献类型：
--
作者：
A. Mulder;J. Broekroelofs;G. Horst;Pieter C. Limburg;G. F. Nelis;Cornelis Kallenberg
67例溃疡性结肠炎患者中有34例（51%）和35例克克隆氏病患者中有14例（40%）通过间接免疫荧光检测到ANCA。除了一种ANCA阳性血清外，所有血清对乙醇固定的中性粒细胞产生核周荧光（P‐ANCA）。在多聚甲醛固定的中性粒细胞上，UC中76%的P - ANCA阳性血清和CD中50%的P - ANCA阳性血清产生细胞质荧光，这表明细胞质抗原确实被相当多的这些血清识别。通过Western blot分析，使用整个中性粒细胞提取物作为底物，46%的UC患者血清和32%的CD患者血清分别与乳蛋白、66/67 kD mol. wt的双峰多肽或63/54 kD mol. wt的双峰多肽发生反应。在自身免疫性肝病和类风湿关节炎患者的P - ANCA阳性血清中观察到相同的反应性模式。这些数据表明，限制性特异性的ANCA并非炎性肠病（IBD）所特有，而是存在于以慢性特发性炎症为特征的多种疾病中。
ANCA were detected by indirect immunofluorescence in 34 out of 67 patients with ulcerative colitis (UC, 51%) and in 14 out of 35 patients with Crobn's disease (CD, 40%). All but one ANCA‐positive sera produced a perinuclear pattern of fluorescence (P‐ANCA) on ethanol‐fixed neutrophils. On paraformaldehyde‐fixed neutrophils 76% of P‐ANCA‐positive sera in UC and 50% of P‐ANCA‐positive sera in CD produced cytoplasmic fluorescenec, indicating that, indeed, cytoplasmie antigens are recognized by a considerable number of these sera. By Western blot analysis using whole neutrophil extract as a substrate 46% of sera from patients with UC and 32%, of sera from patients with CD showed reactivity with either lactolerrin, polypeptides occurring as a doublet of 66/67 kD mol. wt, or polypeptides occurring as a doublet of 63/54 kD mol. wt, respectively. Identical patterns of reactivity have been observed among P‐ANCA‐positive sera from patients with autoimmune liver disease and rheumatoid arthritis. These data suggest that ANCA of restricted specificities are not specific for inflammatory bowel disease (IBD), but are present in diverse conditions characterized by chronic idiopathie inflammation.
中性粒细胞胞浆抗体：原发性硬化性胆管炎和溃疡性结肠炎之间的联系。
影响因子：
29.4
作者：
Duerr,RH;Targan,SR;Landers,CJ;LaRusso,NF;Lindsay,KL;Wiesner,RH;Shanahan,F
通讯作者：
Shanahan,F
影响因子：
14.2
作者：
SAXON, A;SHANAHAN, F;TARGAN, S
通讯作者：
TARGAN, S
影响因子：
158.5
作者：
FALK, RJ;JENNETTE, JC
通讯作者：
JENNETTE, JC