Human Gyrovirus Apoptin shows a similar subcellular distribution pattern and apoptosis induction as the chicken anaemia virus derived VP3/Apoptin.
Human Gyrovirus Apoptin shows a similar subcellular distribution pattern and apoptosis induction as the chicken anaemia virus derived VP3/Apoptin.
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DOI：
10.1038/cddis.2012.34
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发表时间：
2012-04-12
影响因子：
9
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中科院分区：
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鸡贫血病毒衍生蛋白 Apoptin/VP3 (CAV-Apoptin) 具有诱导多种人类癌细胞肿瘤选择性凋亡的重要能力。最近，从人类皮肤拭子中分离出第一个人类陀螺病毒（HGyV）。它显示出与 CAV 显着的结构和组织相似性，并编码 CAV-Apoptin/VP3 的同源物。使用重叠引物，我们构建了与绿色荧光蛋白融合的合成人旋转病毒凋亡蛋白（HGyV-Apoptin），以便将其在各种人类癌细胞系中的凋亡功能与 CAV-Apoptin 进行比较。 HGyV-Apoptin 显示出与 CAV-Apoptin 观察到的相似的亚细胞表达模式，其特点是易位至癌细胞的细胞核，尽管它主要位于正常人细胞的细胞质中。此外，HGyV-Apoptin 或 CAV-Apoptin 在几种癌细胞系中的表达以相当的水平引发细胞凋亡。这些发现表明 HGyV-Apoptin 具有潜在的抗癌作用。
The chicken anaemia virus-derived protein Apoptin/VP3 (CAV-Apoptin) has the important ability to induce tumour-selective apoptosis in a variety of human cancer cells. Recently the first human Gyrovirus (HGyV) was isolated from a human skin swab. It shows significant structural and organisational resemblance to CAV and encodes a homologue of CAV-Apoptin/VP3. Using overlapping primers we constructed a synthetic human Gyrovirus Apoptin (HGyV-Apoptin) fused to green fluorescent protein in order to compare its apoptotic function in various human cancer cell lines to CAV-Apoptin. HGyV-Apoptin displayed a similar subcellular expression pattern as observed for CAV-Apoptin, marked by translocation to the nucleus of cancer cells, although it is predominantly located in the cytosol of normal human cells. Furthermore, expression of either HGyV-Apoptin or CAV-Apoptin in several cancer cell lines triggered apoptosis at comparable levels. These findings indicate a potential anti-cancer role for HGyV-Apoptin.