CpG island clones for chromosome 11p--a resource for mapping and gene identification.
CpG island clones for chromosome 11p--a resource for mapping and gene identification.
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染色体 11p 的 CpG 岛克隆——用于作图和基因鉴定的资源。
DOI：
10.1007/bf00355644
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Gessler,M
中科院分区：
文献类型：
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作者：
Thäte,C;Pongratz,J;König,A;Klamt,B;Tsaoussidou,S;Higgins,M;Shows,T;Jones,C;Gessler,M
构建了人染色体（Chr）11p的ANotI末端片段文库。72个克隆被映射到染色体亚区使用体细胞杂种。克隆检测44个不同的CpG岛，我们已经分离出其中36个粘粒重叠群。从已知的11p13NotI限制性酶切图谱推断，含有NotI位点的11p中每隔一个CpG岛已经在克隆集合中出现。通过序列分析，所有的11p13克隆表现出典型的特征的CpG岛，和跨物种杂交已被检测到在大多数情况下，至少有一个片段。该cDNA作为有价值的连接克隆用于长距离限制性作图。它们还为转录分离程序提供了极好的起始材料，以鉴定染色体11 p上与发育异常和各种肿瘤类型相关的基因。已经用这些克隆中的一些分离了几个转录序列。
ANotI end fragment library has been constructed for human Chromosome (Chr) 11p. Seventy-two clones were mapped to chromosomal subregions by use of somatic cell hybrids. The clones detect 44 different CpG islands, and we have isolated cosmid contigs for 36 of them. Extrapolation from the known 11p13NotI restriction map suggests that every second CpG island from 11p containing aNotsite is already represented in the clone collection. By sequence analysis all of the 11p13 clones exhibit typical features of CpG islands, and cross-species hybridization has been detected with at least one fragment in most cases. The cosmids serve as valuable linking clones for long-range restriction mapping. They also provide excellent starting material for transcript isolation procedures to identify genes on chromosome 11p associated with developmental anomalies and various tumor types. Several transcribed sequences have already been isolated with some of these clones.