TRANSFERABLE CLASTOGENIC ACTIVITY IN PLASMA FROM PERSONS EXPOSED AS SALVAGE PERSONNEL OF THE CHERNOBYL REACTOR
TRANSFERABLE CLASTOGENIC ACTIVITY IN PLASMA FROM PERSONS EXPOSED AS SALVAGE PERSONNEL OF THE CHERNOBYL REACTOR
复制标题
DOI：
10.1007/bf01221035
复制
发表时间：
1994-07-01
影响因子：
3.6
通讯作者：
MERKLIN, L
中科院分区：
文献类型：
--
作者：
EMERIT, I;LEVY, A;MERKLIN, L
染色体断裂因子首先在意外或治疗性辐射的人的血浆中被描述。它们也在原子弹爆炸幸存者中被发现，并在受到辐射后持续存在多年。本研究在 32 名亚美尼亚文职人员的血浆中寻找这些因素，这些人曾于 1986 年在切尔诺贝利原子电站周围担任“取消者”。研究还包括 15 名从前苏联移民到以色列的取消者。参考血浆样本取自埃里温亚美尼亚血液中心的 41 名献血者。在健康捐献者的血液培养物中测试了样品的断裂活性。清算人的大部分结果都超过了未暴露的参考样品的结果。来自第一组亚美尼亚组的样品具有较高的平均辐射剂量（0.6+/-0.6 Gy），比来自暴露于0.2+/-0.2 Gy 的第二组的样品具有更强的染色体断裂性。测试培养物中的畸变数分别为 17.9+/-2.9% 和 10.5+/-3.8%，而暴露于亚美尼亚献血者参考超滤液的培养物中的畸变数为 5.7+/-3.2%。来自以色列清算者的样本还导致异常率显着增加（14.0+/-3.9% 异常细胞）。断裂活性经常被超氧化物歧化酶抑制，这表明辐射诱导的断裂因子的染色体损伤作用是通过超氧自由基的中介发挥的，正如已知的不同来源的断裂因子一样。
Clastogenic factors were first described in the plasma of people who had been accidentally or therapeutically irradiated. They were found also in A-bomb survivors, where they persisted for many years after the irradiation. The present study searched for these factors in the plasma of 32 civil workers from Armenia, who had been engaged as ''liquidators'' around the Chernobyl atomic power station in 1986. It also included 15 liquidators who had emigrated from the ex-Soviet Union to Israel. Reference plasma samples were obtained from 41 blood donors from the Armenian Blood Center in Yerevan. The samples were tested for their clastogenic activity in blood cultures from healthy donors. The majority of results from the liquidators exceeded those from the unexposed reference samples. The samples from the first Armenian group, with the higher average irradiation dose (0.6+/-0.6 Gy), were more clastogenic than those from the second group exposed to 0.2+/-0.2 Gy. The number of aberrations in the test cultures was 17.9+/-2.9% and 10.5+/-3.8% respectively, compared to 5.7+/-3.2% in the cultures exposed to the reference ultrafiltrates from Armenian blood donors. The samples from the Israeli liquidators also induced significantly increased aberration rates (14.0+/-3.9% aberrant cells). The clastogenic activity was regularly inhibited by superoxide dismutase, indicating that the chromosome-damaging effects of radiation-induced clastogenic factors are exerted via the intermediation of superoxide radicals, as is known for clastogenic factors of different origin.