Effect of Total Alkali in Leonuri Herba on rat ear acne model of serum IL-6 level, Thymus and Spleen Tissue Morphology.
Effect of Total Alkali in Leonuri Herba on rat ear acne model of serum IL-6 level, Thymus and Spleen Tissue Morphology.
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DOI：
10.1016/j.sjbs.2017.01.047
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发表时间：
2017-03
影响因子：
4.4
通讯作者：
Miao MS
中科院分区：
文献类型：
--
作者：
Li Y;Xi P;Wang T;Miao MS
探讨益母草总碱（TALH）对大鼠耳痤疮模型血清IL-6水平及胸腺、脾脏组织形态的影响。将大鼠分为高、中、低剂量TALH组（200 mg kg−1、100 mg·kg−1、50 mg·kg−1）、丹参酮组（360 mg·kg−1）、模型组和空白组。各组大鼠每天给药1次，连用14 d。第1天，空白组大鼠同时胃内注射生理盐水，耳廓皮内注射无菌生理盐水。其余各组大鼠（模型组灌洗生理盐水）皮内注射表皮葡萄球菌备用菌液。注射后连续5天通过测量大鼠耳廓厚度计算大鼠耳廓肿胀率。末次给药后1 h，取眼球血离心分离血清，测定血清IL-6水平；按形态学标准观察胸腺和脾脏组织切片。与空白组比较，模型组大鼠血清IL-6水平显著升高，胸腺组和脾脏组血清IL-6水平显著降低，提示大鼠耳痤疮模型成功。与模型组比较，TALH组大鼠血清IL-6水平明显降低，胸腺皮质明显增厚，脾脏切片明显增多，胸腺和脾脏淋巴细胞明显增多。TALH组大剂量组疗效最好，并随剂量和疗效的增加而增强。TALH治疗痤疮可能与降低大鼠耳痤疮模型血清IL-6水平及对胸腺和脾脏萎缩的抵抗有关。
To investigate the effect of Total Alkali in Leonuri Herba (TALH) on rat ear acne model of serum IL-6 level, Thymus and Spleen Tissue Morphology. The rats were divided into the TALH group at high, medium and low doses (200 mg kg−1, 100 mg·kg−1, 50 mg·kg−1), the tanshinone group (360 mg·kg−1), model group and the blank group. All the rats were administrated once a day for 14 days. At the same time on the first day, the rats in the blank group were injected normal saline in the stomach and also had intradermal injection of sterile saline in the auricle. The rats in other groups (model group were lavaged with physiological saline) were injected intradermally auricle spare Staphylococcus epidermidis bacteria liquid. After the injection, the rat ear swelling rate was calculated for 5 consecutive days in terms of rat auricle thickness measurement. 1 h after the last administration, the eyeball blood was removed, centrifuged to separate serum, and measured to be at serum IL-6 level; The tissue sections of thymus and spleen were observed according to morphology criteria. Compared with the blank group, the level of IL-6 in serum of the rats in the model group was remarkably higher, and those in the thymus and spleen groups were remarkably lower, indicating the rat ear acne model is successful. Compared with the model group, the rats in the TALH group can remarkably make serum IL-6 level decrease, causing significant thymus cortical thickening and increased spleen sections, and remarkably increasing the lymphocytes of thymus and spleen. The effect of large dose in the TALH group is the best and increases with the increase of the dose and curative effect. The TALH treatment of acne may be related to the reduction of serum IL-6 levels and the resistance of the atrophy of the thymus and spleen on the rat ear acne model.