VIROLOGICAL MARKERS IN THE CEREBROSPINAL-FLUID FROM HIV-1-INFECTED INDIVIDUALS
VIROLOGICAL MARKERS IN THE CEREBROSPINAL-FLUID FROM HIV-1-INFECTED INDIVIDUALS
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DOI：
10.1097/00002030-199112000-00002
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发表时间：
1991-12-01
期刊：
影响因子：
3.8
通讯作者：
HURAUX, JM
中科院分区：
文献类型：
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作者：
BUFFET, R;AGUT, H;HURAUX, JM
我们分析了127例脑脊液（CSF）从118 HIV-1感染者在不同阶段的感染。鞘内抗体合成是明显的，在23个样本测试，更频繁地针对艾滋病毒比对风疹病毒，单纯疱疹病毒，水痘带状疱疹病毒或巨细胞病毒。在127份CSF样本中，仅14%分离出HIV，但在CSF配对的血液样本中，分离出HIV的比例为82%。在12%的CSF样本和44%的配对血浆样本中检测到HIV抗原。使用聚合酶链反应（PCR）分析的20份标本在75%的CSF标本中检出前病毒DNA。脑脊液中病毒回收率低既不是培养前冷冻标本所致，也不是治疗所致。相反，从CSF中分离的病毒与CSF细胞计数显著相关。CSF中的病毒分离和抗原检测与疾病控制中心疾病分期或外周血CD 4+淋巴细胞计数无关，而病毒血症与低CD 4+淋巴细胞计数显著相关。此外，脑脊液中的病毒分离和抗原检测与亚急性HIV脑炎的症状无关，表明这些标志物在诊断HIV特异性神经系统并发症中没有潜在价值。PCR在这一领域的价值值得进一步研究。
We analysed 127 specimens of cerebrospinal fluid (CSF) from 118 HIV-1-infected individuals at different stages of infection. Intrathecal antibody synthesis was evident in 23 samples tested and was more frequently directed against HIV than against rubella virus, herpes simplex virus, varicella zoster virus or cytomegalovirus. HIV was isolated from only 14% of the 127 CSF specimens, but from 82% of CSF-paired blood samples. HIV antigen was detected in 12% of CSF specimens and 44% of paired plasma samples. Twenty specimens analysed using the polymerase chain reaction (PCR) detected proviral DNA in 75% of CSF specimens. The low rate of virus recovery from CSF was caused by neither the freezing of specimens prior to culture nor therapy. In contrast, virus isolation from CSF was significantly associated with CSF cell count. Virus isolation and antigen detection in CSF were not correlated with either the Centers for Disease Control disease stage or the peripheral CD4+ lymphocyte count, whereas viraemia was significantly associated with a low CD4+ lymphocyte count. Moreover, virus isolation and antigen detection in CSF were not associated with symptoms of subacute HIV encephalitis, suggesting that these markers are not of potential value in the diagnosis of HIV-specific neurologic complications. The value of PCR in this field merits further investigation.