Characterization of laminaran and a highly sulfated polysaccharide from Sargassum fusiforme
Characterization of laminaran and a highly sulfated polysaccharide from Sargassum fusiforme
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DOI：
10.1016/j.carres.2013.12.009
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发表时间：
2014-02-19
影响因子：
3.1
通讯作者：
Zhang, Quanbin
中科院分区：
文献类型：
--
作者：
Jin, Weihua;Zhang, Wenjing;Zhang, Quanbin
羊栖菜粗多糖(HFS)用0.1M盐酸提取，阴离子交换层析分离得到HFS-1、HFS-2和HFS-3三个组分。经化学分析，HFS-1由海带多糖组成，HFS-2由海藻酸盐和硫酸基杂多糖组成，HFS-3主要由硫酸基半乳糖组成。核磁共振谱表明，HFS-1是由可溶性的海带多糖组成的，链的末端是β-D-葡萄糖残基。相比之下，HFS-2的光谱仍然很复杂，即使在去除了大部分藻酸盐之后也是如此。此外，HFS-3可能含有C-2硫化的3-连接岩藻聚糖硫酸酯、C-2硫化的6-连接半乳聚糖和2-硫化Fuc在C-4的支化、C-2硫化的交替Gal和Fuc或C-2硫化的交替(Gal)(N)和(Fuc)(N)的骨架的半乳糖岩藻糖胶。此外，HFS-3还含有少量岩藻糖苷甘露聚糖和木聚糖。(C)2013爱思唯尔有限公司。保留所有权利。
The crude polysaccharide (HFS) from Sargassum fusiforme (Hizikia fusiforme) was extracted using 0.1 M HCl and was fractionated by anion-exchange chromatography into three fractions: HFS-1, HFS-2, and HFS-3. Based on the chemical analysis, HFS-1 was composed of laminaran, HFS-2 was a mixture of alginate and sulfated heteropolysaccharides, and HFS-3 was primarily composed of sulfated galactofucan. The NMR spectra revealed that HFS-1 was composed of a soluble laminaran with chains that are terminated by beta-D-glucose residues. In contrast, the spectra obtained for HFS-2 were still complex, even after most of the alginate was removed. In addition, HFS-3 might contain 3-linked fucan sulfated at C-2, 6-linked galactan sulfated at C-2 and branched at C-4 by 2-sulfated Fuc, and galactofucan with a backbone of either alternating Gal and Fuc sulfated at C-2 or alternating (Gal)(n) and (Fuc)(n) sulfated at C-2. Moreover, HFS-3 also contained small amounts of fucoglucuronomannan and xylan. (C) 2013 Elsevier Ltd. All rights reserved.