A gravity-actuated technique for flexible and portable microfluidic droplet manipulation
A gravity-actuated technique for flexible and portable microfluidic droplet manipulation
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DOI：
10.1007/s10404-010-0611-6
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发表时间：
2010-10-01
影响因子：
2.8
通讯作者：
Luo, Guoan
中科院分区：
文献类型：
--
作者：
Zhang, Kai;Liang, Qionglin;Luo, Guoan
本研究描述了一种基于重力驱动技术的廉价、鲁棒的微流控液滴操纵装置。该设备主要由一个转盘和一些一次性输液器组成。微通道内油相和水相的运动是可控的。利用转盘控制液滴长度，精确生成不同大小的液滴体积。包括按需采样、可控大小和可控数量的液滴生成、不同大小和含量的液滴融合、甚至液滴停止等一系列基本功能单元。这些功能可以很好地满足基于微流体的常规生物/化学分析、细胞研究和高含量药物筛选的需求。与一般的液滴操作系统相比，该技术具有廉价、灵活和便携的优点，该系统包含精密的注射泵和复杂的微机械/气动阀。此外，该装置可以作为户外和偏远地区的便携式液滴操作工具，因为它不需要电源，在任何地方都可以方便地安装。
In this study, an inexpensive and robust microfluidic droplet manipulation device based on gravity-actuated technique is described. The device is mainly composed of a turntable and some disposable infusion sets. The movement of oil-phase and water-phase in the microchannel is controllable. Droplet volume of different sizes is precisely generated by controlling droplet length using the turntable. A series of essential functional units have been demonstrated including on-demand sampling, droplets generation of controllable size and controllable number, droplets fusion of different sizes and contents, even droplets-stopping. These functions can well meet the requirements of routine droplet-based microfluidic biological/chemical assays, cell research, and high content drug screening. The presented technique shows unique advantages in inexpensiveness, flexibility, and portability when compared with general droplet manipulation systems containing precision syringe pump and Complicated micromechanical/pneumatic valve. Furthermore, this device can be used as a portable droplet manipulation tool in outdoor and remote areas, because it does not need electrical source and is very facile to set up in any place.