REGULATION OF ESCHERICHIA-COLI K5 CAPSULAR POLYSACCHARIDE EXPRESSION - EVIDENCE FOR INVOLVEMENT OF RFAH IN THE EXPRESSION OF GROUP-II CAPSULES
REGULATION OF ESCHERICHIA-COLI K5 CAPSULAR POLYSACCHARIDE EXPRESSION - EVIDENCE FOR INVOLVEMENT OF RFAH IN THE EXPRESSION OF GROUP-II CAPSULES
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DOI：
10.1111/j.1574-6968.1994.tb07267.x
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发表时间：
1994-11-15
影响因子：
2.1
通讯作者：
ROBERTS, IS
中科院分区：
文献类型：
--
作者：
STEVENS, MP;HANFLING, P;ROBERTS, IS
大肠杆菌K5抗原的表达被用作模型系统，以研究已知的基因表达调节因子对大肠杆菌中II组荚膜产生的作用。杆菌只有rfaH基因的突变对K5抗原的产生有影响，在37 ℃下消除了任何可检测到的胶囊的表达。所研究的突变均未在18摄氏度下诱导被膜表达。一个序列，称为JUMPstart，发现在II组胶囊基因簇和上游的一些多糖生物合成基因在肠道细菌是同源的RfaH调节操纵子中发现的序列。这可能表明RfaH对这些多糖生物合成基因的调节具有共同模式。
Expression of the Escherichia coli K5 antigen was used as a model system to study the role of known regulators of gene expression on production of group II capsules in E. coli. Only mutations in the rfaH gene had an effect on production of the K5 antigen, abolishing the expression of any detectable capsule at 37 degrees C. None of the mutations studied induced capsule expression at 18 degrees C. A sequence, termed JUMPstart, found in group II capsule gene clusters and upstream of a number of polysaccharide biosynthesis genes in enteric bacteria is homologous to sequences found in RfaH regulated operons. This may indicate a common mode of regulation of these polysaccharide biosynthesis genes by RfaH.