Distinct localization of collagenase and tissue inhibitor of metalloproteinases expression in wound healing associated with ulcerative pyogenic granuloma.
Distinct localization of collagenase and tissue inhibitor of metalloproteinases expression in wound healing associated with ulcerative pyogenic granuloma.
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DOI：
10.1172/jci116073
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发表时间：
1992-11
期刊：
影响因子：
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通讯作者：
U. K. Saarialho-Kere;E. S. Chang;H. Welgus;William C. Parks
中科院分区：
文献类型：
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作者：
U. K. Saarialho-Kere;E. S. Chang;H. Welgus;William C. Parks
为了研究金属蛋白酶在与伤口愈合相关的组织重塑中的作用，我们使用原位杂交技术定位了化脓性肉芽肿样本中胶原酶和金属蛋白酶组织抑制剂（TIMP）的表达。在所有溃疡病变前缘附近的基底角化细胞中检测到胶原酶mRNA的强杂交，但在没有溃疡的样本中未见胶原酶mRNA。与胶原酶表达位点不同，TIMP mRNA在基质细胞和增生血管周围细胞中检测到。健康皮肤表皮未发现胶原酶mRNA，但偶尔基质细胞含有胶原酶或TIMP mRNA，毛囊和皮脂腺中检测到TIMP mRNA。我们的研究结果表明，损伤表皮附近的基底角化细胞在基质重塑中起着至关重要的作用，其作用远远超过相邻或下层的真皮成纤维细胞。此外，一些报道表明，TIMP可能在血管生成中起作用。最后，与其他模型表明胶原酶和TIMP蛋白由相同细胞分泌的结果相反，我们的数据还表明这些蛋白可以在体内相互独立地产生。
To examine the role of metalloproteinases in tissue remodeling associated with wound healing, we used in situ hybridization to localize the expression of collagenase and tissue inhibitor of metalloproteinases (TIMP) in samples of pyogenic granuloma. Strong hybridization for collagenase mRNA was detected in basal keratinocytes near the advancing edge of all ulcerative lesions, but no collagenase mRNA was seen in samples without ulceration. Distinct from the sites of collagenase expression, TIMP mRNA was detected in stromal cells and in cells surrounding proliferating vessels. No collagenase mRNA was found in the epidermis of healthy skin, although occasional stromal cells contained collagenase or TIMP mRNAs, and TIMP mRNA was detected in hair follicles and sebaceous glands. Our results suggest that basal keratinocytes adjacent to wounded epidermis are critically involved in matrix remodeling, much more so than adjacent or underlying dermal fibroblasts. Furthermore, as several reports have suggested, TIMP may play a role in angiogenesis. Finally, in contrast to findings from other models which indicate that collagenase and TIMP proteins are secreted by the same cells, our data also demonstrate that these proteins can be produced in vivo independently of each other.